Analysis For Antibacterial Activity Of Bioactive Compounds In Sponge, Etc By Interaction Assay With Bacteria

The main hindrances to developing natural medicines are scientific interpretation of the pharmacology and mechanisms of action of the active ingredients. This paper summarizes recent progress in marine drugs, TCMs and drug interaction assay with cells. In this work, an analysis strategy was established based upon the interaction between drugs and cells. And the binding of bioactive components with bacteria were characterized, correlation of antibacterial properties and binding degree for active components were preliminarily discussed.We investigated the UV-Vis spectra and the proportion of the mobile phase for a variety of active ingredients of the two kinds of traditional Chinese Medicine and sponge by HPLC. In addition, a secondary amine compound was identified by HPLC-MS, UV-Vis spectroscopy, MS and Infra-red spectroscopy. The qualitative and quantitative analysis were performed by HPLC, UV-Vis for Andrographolide, Chlorogenic acid, Wogonin in the 95% ethanol extract of Andrographis panicu Lata, and Chlorogenic acid, Luteolin in the 95% ethanol extract of Honeysuckle.We adopted the Centrifugal Ultrafiltration(CFU)-HPLC method to investigate activities of the binding for the active ingredients from Andrographis panicu Lata, honeysuckle and sponge with E. coli, Staphylococcus aureus to establish fingerprint analysis method of interaction in vitro for E. coli and Staphylococcus aureus. The binding activity of wogonin from the extract of Andrographis panicu Lata and E. coli, Staphylococcus aureus is high, the binding activity of Chlorogenic acid in the single standard and mixed standards and E. coli is very low, and the binding activity of Chlorogenic acid from the mixed standards of Chlorogenic acid and luteolin and Staphylococcus aureus is extremely low. Under the experimental conditions, the binding rate between sponge and E. coli cells is in the rate of 5.3%- 18%, and the binding rate between sponge and Staphylococcus aureus cells is in the rate of 4.7%- 14.4%.