The Effects Of Limited Fluid Resuscitation On The Function Of Pancreatic Islet Cells

Purpose: Limited fluid resuscitation(LFR) is a better method for the treatment to uncontrolled hemorrhagic shock( UHS)in recent years. However, less research for the influence on the function of pancreatic islet cells has been done. The contrastive study is desi gned to explore the influence of LFR on pancreas islet function through observing the change of glucagon(GG) and C-Peptide(CP) in serum during the therapeutic process and comparing the data by using statistical methods while investigating the impaction of LFR on the function of pancreas islet. We expect that the research may provide clinical data for the security of LFR and provide a reference for clinical treatment. Method: We collected 60 patients of uncontrolled hemorrhagic shock in Linyi People’s Hospital who were hospitalized from March to December in 2014. They were randomly divided into LFR group and AFR group and treated with fluid resuscitation. The group LC as a study group and the group OC as a Control group.We collected their blood samples at three time periods, namely the time of hospitalization(T1), pre-operation(T2) and 3 hours after operation(T3). The concentrations of CP and GG were measured by means of radioimmunoassay and compared with statistical approach. Result: 1. The change of glucagon level in two groups: The concentrations of GG and CP at the time of pre-operation(T2) are not significantly different from hospitalization(T1)(P>0.05). However, the concentrations of GG at the time of 3 hours after operation(T3) in LFR group is obviously lower than AFR group(P<0.05). 2. The change of C-peptide level in two groups: The concentrations of GG and CP at the time of pre-operation(T2) are not significantly different from hospitalization(T1)(P>0.05). However, the concentrations of CP at the time of 3 hours after operation(T3) in LFR group is obviously lower than AFR group(P<0.05). 3. There is no significant difference for age, gender, hospital admission time of injury, preoperative time, volume of infusion prior to admission, ISS between two groups. Conclusion: While two resuscitation methods having obvious influence on the function of pancreatic islet cells, LFR has a less influence on the function of islet cell and higher security in clinical application than AFR. LFR has certain advantages compared to AFR in therapy and it should be a much safer treatment method.