The Effect Of γ Ray-induced Nerve Cell Damage On Immune Cell

Ionizing radiation can cause damage to the organism’s genetic material, which would cause cell necrosis and apoptosis. The body would be damaged by serious ionizing radiation, which often appeared in the hospital for the radiotherapy treatment of tumor patient and the cosmic rays on the space. As one of the vital organs, the brain structure is complex and powerful. When the brain is damaged by radiation, often accompany a series of inflammatory reaction. Because organisms are too complex, so using cell lines established cell model of neuroimmune interaction in this article and explore what the effect for nerve cells which was damaged by radiation to the immune cells. In the article, using the SH-SY5 Y cells(the nerve cells model in vitro), U87 MG(glial cells model in vitro), THP 1/U937(mononuclear macrophage model in vitro) and Jurkat(T cells model in vitro) cells build neuroimmune interaction model in vitro. While co-cultured, Nerve cells SH-SY5 Y mixed with U87 MG cells, after radiation then culture for 24 hours, 48 hours and 72 hours after radiation. Collecting the co-culture medium and giving it to the immune cells. 24 hours later, collecting the immune cells, and testing the immune cells in the experiment. At first,we chose the condition which is cultured for 72 hours and irradiated by 10 Gy for THP-1 cell,and selected 7Gy and 24 hours cultured time for Jurkat.There are four experiment for THP-1 cells. The first one was BrdU proliferation test, the second one was immunofluorescence staining, the third one was an FACS for cell surface glycoprotein which express on activated macrophages, the fourth one was chemotactic experiment. For Jurkat cells, there were just cell counting experiment, cycle flow detection and annexin V-FITC flow detection. From the experiment, we learned that co-culture cells damaged by radiation in 10, 15 Gy would inhibit proliferation and promote on THP-1 activation, and can produce chemotaxis to THP 1 cells. Co-culture cells damaged by radiation would inhibit the proliferation of Jurkat cells.Therefore, we concluded that when the nervous system damage exposure to the radiation, can produce chemokines activating mononuclear differentiation to macrophages and raise more immune cells to the injury, but at this point, the adaptive immunity, such as, T cell proliferation is inhibited.