Study On The Secondary Metabolites Of Two Marine Coral-derived Microorganisms

The study was conducted to investigate secondary metabolites of coral-associated marine actinomycete 11492 and bacterium 11593, which showed cytotoxic activity in early screening. Isolation and purification were carried out by using column chromatography over silica gel, sephadex LH-20, ODS column chromatography and other chromatographic techniques. Nine compounds were identified as 2’-deoxyadenosine(1), 2’-deoxythymidine(2), 2’-deoxyuridine(3), uridine(4), 1-O-hexadecanoyl-3-O-β- D-galactopyranosyl-glycerol(5), p-hydroxybenzoic acid(6), Nb-acetyl-2, 3-dihydro-3-hydroxy-2-oxotryptamine(7), N-acetyltryptamine(8) and N-acetyltyramine(9) by modern spectroscopic methods(NMR, MS and IR). Among them, compounds 1~5 was isolated from the strain 11492 and compounds 6~9 was obtained from the strain 11593. Compound 7 is a new natural product. It was found that compound 5 exhibited weak cytotoxic activity against RAW264.7 with IC50 value of 10.9 μM but other compounds showed no cytotoxic activity.