Induce Differentiation Of Human Gingival Fibroblasts Into Osteoblasts In Vitro

The stem cells are more and more widely used in the periodontal tissue engineering. However, various stem cells have different defects such as hard to get, body damage, difficult to culture in vitro. Gingival fibroblasts are adult stem cells which have active abilities to update themselves, easily obtained, less damage to the body, high survival rate in vitro, with a certain research value. The topic is about how to culture and induce gingival fibroblasts in vitro, and to detect the osteogenic potential of gingival fibroblasts.Part Ⅰ Induce differentiation of human gingival fibroblasts into osteoblasts in vitroBackground:Periodontal tissue engineering is a hotspot of research about repairment of periodontal tissue defects due to periodontitis. Gingival fibroblasts get more and more attentions from people because they may become seed cells for periodontal tissue engineering.Objective:To culture human gingival fibroblasts (GFs) in vitro, induce them to differentiate into osteoblasts, and investigate their differentiation ability.Methods:Human GFs were extracted. The passaged GFs were cultured for differentiation with aid of some chemical drugs. The activity of alkaline phosphatase and calcific nodule were analyzed.Results:Compared with control group, human GFs of experimental group showed enhanced expressions of alkaline phosphatase and produced more calcific nodules after treatment with some chemicals.Conclusion:Under certain culture condition and inducement, human GFs might have application prospect in the periodontal tissue regeneration.Part Ⅱ Controlled micro-nano-pattern induce human gingival fibroblasts to osteoblast-like cellsBackground:The surface pattern of the biological material is with great significance in the study of tissue engineering and cell biology, one of the most commonly used methods to study the interaction between the material surface and the cell.Objective:To study the effect of the size of the micro-pattern on human gingival fibroblasts behavior, to prove that the patterned surface can effectively control the morphology of the cells.Methods:To produce highly selective two-dimensional chemical micro pattern on surface of glass. Human gingival fibroblasts were seeded on the micro-patterned surface of two sizes. Cell morphology was observed.Results:With the same space of 250μm, compared to 30μm ×30μm micro-pattern,90μm×90μm one is more conducive to the growth and differentiation of human gingival fibroblasts.Conclusion:Human gingival fibroblasts spread better on the pattern of large-size than on the pattern of small size.