Chem-and Photo-active Nanocompostite Based On Functionalized Graphene For Cancer Combination Therapy

In recently years, tumor probability has become more increasingly prominent, and the methods of treating cancer have become been increasingly diversified. The traditional therapy: chemotherapy is currently the most widely used treatment, but its less effectiveness and side effects troubles in the clinical treatment. And Photodynamic therapy is a new means of treating cancer, which attracts more and more attentions, due to its validity and uniqueness. but also it faces a huge challenge, because the photosensitizers which can produces singlet oxygen, are mostly hydrophobic, difficult to arrive at the tumor. It is necessary and key to build a safe and can carry out a variety of different mechanisms of drugs. Combining two treatment, it can further enhance the effect of cancer.In this paper, I constructed the poly-lysine modified graphene(G-PLL), while carrying a chemotherapy drug doxorubicin(DOX) and a photosensitive drug zinc phthalocyanine(ZnPc), combining chemotherapy and photodynamic therapy to inhibit the growth of various tumor cells G-PLL carrier has good biocompatibility and stability: the size of it is about 200 nm and Zeta potential of up to +29.25 mV. G-PLL can carry alone doxorubicin(drug loading:110%) and photosensitizer zinc phthalocyanine(drug loading:59%).Further it is capable of carrying two drugs simultaneously to form a nanocomposite G-PLL/DOX/ZnPc which has good stability and dispersibility. Through the biological study, under irradiation at a wavelength of 670 nm, it can produce singlet oxygen successfully. The results showed that, nanocomposite can be swallowed in the variety tumor cells. Based on the inhibition rate of 50% CI50 synergy index were 0.07(HeLa),0.13(MCF-7) and 0.13(B16), the nanocomposite has strong chemotherapy and photodynamic therapy synergie effect in multiple tumor cells.This study shows that the drug G-PLL/DOX/ZnPc nanocomposite has a good synergistic inhibition of tumor cell proliferation and promote apoptosis of tumor cells, offering a new way of cancer treatment for cancer combination therapy.