The Expression Of The Hemo Oxgenase-1 And IL-8 In Pations With Lung Tissue For One-lung Ventilation For Thoracoscopic Lobectomy Of Ulilnastatin

Background:In clinical anesthesia work, one lung ventilation is an anesthesia way for patients to ventilate with non collapsed lungs,the patients must use double-lumen endotracheal intubation or bronchial cccluder. The main purpose is to isolate the non- ventilated lung, Prevent blood, airway secretions, such as falling into the ventilated lung tissue, Create a good environment for the surgeon surgery. OLV is mainly used for thoracic surgery, minimally invasive esophageal surgery, minimally invasive cardiac surgery, major vascular surgery and so on. With the rapid development of minimally invasive surgical techniques, the indications for surgery is continued to expand. Single lung ventilation requirements are also increasing. This study is to observe the expression of the hemo oxgenase-1 and IL-8 in patients with lung tissue for one-lung ventilation for thoracoscopic lobectomy of ulilnastatin.Purpose and significance:OLV is a modern cardiothoracic surgery anesthesia commonly used anesthetic management,this non-physiological way ventilation can cause acute lung injury. In the single- lung ventilation(OLV) process, Cause pulmonary shunting and ventilation / perfusion ratio decreased. Meanwhile hypopnea state can cause cell damage and ischemic and hypoxic injury, Hypoxic pulmonary side in the recovery after the blood supply further exacerbate cell injury. Under normal physiological conditions, in the endogenous antioxidant system can promptly remove excess oxygen free radicals generated. However, under pathological conditions, due to excessive reactive oxygen species that can not be cleared in time, the balance is broken, causing a severe oxidative stress, leading to cell dysfunction. Coupled with the mechanical stimulation caused by surgery, can cause inflammation of the lungs, induce or aggravate lung injury. With extensive thoracoscopic technique for OLV increasingly demanding. Long collapsed lung tissue hypoxia and mechanical stimulation surgical procedures, can cause collapse of the lung tissue damage, how to reduce the damage caused by lung collapse, is increasingly being valued by everyone.Research objects and materials:Select 60 patients come from the First Affiliated Hospital of Henan University from2012 to 2014,the patients were given one-lung ventilation in thoracoscopic lobectomy. 32 cases were male,28 cases were female, Ages range from 55 years to 70 years.The weight is 50-75 kg. All of the subjects and follow-up data are complete, All of the subjects were first treatment, Never received chemotherapy, radiation therapy, immunotherapy and other treatments, All patients had signed informed consent of patients or their families. Required medicines : UTI, midazolam injection along with benzene sulfonamide atracurium, sufentanil, remifentanil, etomidate, sevoflurane. Required equipment: Ohmeda anesthesia machine, Bis depth of anesthesia monitors, electronic syringe pump, blood gas analyzer, bronchoscopy, double-lumen endobronchial tube, Philips MP60 multi-parameter monitor, Siemens refrigerator, electric bath temperature water bath, Sigma low-speed centrifuge, Bole 680 microplate reader, UV spectrophotometer(UV-2450).Methods60 cases thoracoscopic lobectomy were randomly divided into 2 groups:NS groups(A groups), ulilnastatin groups(B groups),. 30 min administration before induction of anesthesia. Taking a normal lung tissue as a control group(d group) before OLV.Take two piece of normal lung tissue to observe after the lung was cut.one piece of lung tissue was observe by election microscrape,the others to detect the expression of HO-1.Two groups of pations respectively after induction of anersthesia(T1),OLV45min(T2), OLV90(T3) and postperative2h(T4) take 3ml peripheral blood samples to determine the conentration of IL-8.ResultsThe lung tissue structure of d groups is clear and complete,the alveolar wall is thin.the alveolar septa is normal. The lung tissue structure of B groups is still clear and complete, thin alveolar wall, alveolar septa normal alveolar ableeding, edema. The lung tissue stucture of groups A is unclear.alveolar wall thickening,visible alveolar hemorrhage,edema.alreolar septa is abnormal.Compared with groups A,the HO-1 of groups B upregulated(P<0.05).To compare with T1,the serum IL-8 of two groups of patiens were significantly increased,(P<0.05).Compared with groups A,the concentation of IL-8 reduce in T3,T4 phase.ConclusionsUlilnastatin OLV for thoracoscopic lobectony in pations with lung tissue exprssion of HO-1 upregulatde,lower concentrations of IL-8.