Inhibition Of Wenyang Qingre Huatan Formula And Its Seperated Prescriptions On PI3K Signaling In Pituitary Tumors Cell AtT-20

Objective This paper introduces the effective prescription of inhibited growth of pituitary adenoma, which consists of common Chinese medicine for pituitary adenoma. With the observation on the effect of the prescription, the paper discusses the mechanism of the recipe and disassembled recipe of tonify effect, dispelling dampness, and activating blood for pituitary adenoma. The article also discusses the situation that Chinese medicine pathology provides feasible recipes in treating diseases like pituitary adenoma with Chinese medicine.Methods The paper makes frequency analysis on documents of Chinese medicine treatment for pituitary adenoma in CNKI between June,1979 and March, 2014. After preliminary screening of the medicines,269 herbal medicines was selected and dealt with 95% alcohol immersion extraction. I Started to make related medicines on the seventh day. Pituitary adenoma At T-20 cell was treated with 100um/ml concentration and then observed the changes of morphology under inverted microscope. 48 hours later, drugs that inhibit At T-20 cell was selected with MTT method. On the basis of orthogonal design, the recipe with best effect was formulated. The effect of temperature and heat clearing and removing phlegm and removing prescription on At T-20 cycle of cells was determined by flow cytometry.At the same time, the paper describes the results of observing these medicines effect on At T-20 with the method of western blot, discusses the relations between Chinese pathogenesis, and At T-20 growth signal transduction, and pursues the effective prescription for pituitary adenoma.Results 1 The recipe and separated recipe of promoting yang, hotblcok and resolving phlegm has obvious inhabited effect on At T-20 cell.2 Under normal circumstances, the pituitary tumor At T-20 cells were round, the drug was 100 g/ml.And we found the cell of blank control group membrane contour clear, the growth is in good condition and full shape filling, a circular closely into the group, the cells are evenly distributed, value-added exuberant.The dosing group has obvious inhibitory effect on the growth of pituitary tumor cells At T-20, with slow growth and small number. Part of the cell membrane shrinkage foam, small cell shrinkage and translucent, cell debris increased. Turn round and cell shrinkage, cell gap regularity increases, floating dead cells are more. 3 Wenyang Qingre Huatan Decoction and its disassembled prescriptions can effectively inhibit the growth of At T-20 cells, the whole formula group inhibition has the strongest effect, inhibition rate increased with the increase of drug concentration, time, inhibition of cell growth showed a dose- effect and time- effect dependence. 4 The temperature and clearing heat and resolving phlegm and removing phlegm and removing phlegm and removing prescription have obvious effect on the cell cycle of pituitary tumor cells At T-20. And compared to the control group, Wenyang Qingre Huatan Decoction and its disassembled role group ratio of G0 / G1 phase cells increased, whole group was significantly increased; Wenyang Qingre Huatan Decoction and its disassembled Party group of cells in S phase was decreased, which the whole Party and warming Yang group decreased larger proportion; Warming Yang Qingre Huatan formula and its disassembled role group G2 / M phase cell ratio increased, the phlegm group G2 / M phase cell ratio increased significantly. 5 At T-20 can detect the expression of PDGFR-α, PI3 K, AKT and NFk B proteins in different degrees of pituitary tumor cells. 6 When IC50 drug dose was used, the expression levels of PDGFR, AKT, PI3 K and NFKB pathway were down regulated by the warming yang and clearing heat and resolving phlegm and both parties.Conclusions 1 Warming yang and clearing heat and resolving phlegm and its three prescriptions for At T-20 growth are inhibitory, the effect of the whole group is best, warming Yang, clearing heat, and resolving phlegm three has synergistic effect. 2 The effect of the inhibition of At T-20 by Warming Yang and clearing heat and resolving phlegm and removing phlegm and removing phlegm and removing phlegm and removing phlegm and removing phlegm is the time dependent effect of the dose and time dependence. 3 The main mechanism of the inhibition of the growth of the tumor cells by the warming yang and clearing away heat and resolving phlegm and removing phlegm and removing phlegm and removing phlegm and removing the side of the tumor cells is the S phase of the cell cycle.The effect of the strength and the weak and the strong or weak for the whole group > warming Yang Group > clearing heat group > phlegm group. 4 The pathogenesis of pituitary adenoma is related to the enhancement of PDGFR-PI3K-AKT-NFKB signaling pathway. 5 The expression levels of PDGFR, PI3 K, AKT and NFKB were down regulated by temperature and heat clearing and clearing heat and resolving phlegm and both parties, and the proliferation of tumor cells was inhibited. 6 The effect of disproof, confirmed in pituitary tumor lesions, Yang deficiency, phlegm and toxin, PDGFR, PI3 K, AKT, NFKB alpha expression and signal transduction enhanced relationship. 7 Through literature analysis and experimental results we can found toxic heat, phlegm condensation of Yang deficiency disease in the pituitary tumor diseases.