The Reseach About The Correlation Of Osteoporosis And CMKLR1 Gene Knock Out On ORX Mice Arthritis

Objective:1. To study the CMKLR1 knockout of male mice bone marrow mesenchymal stem cells(BMSC) between the influence of the ability of differentiation.2. To clari fy CMKLR1 knockout effects on male mice bone metabolism.Methods:The experiment consists of in vivo and in vitro experiments.For in vitro experi ments, we mainly cultivate CMKLR1 separation- ko sources and sources of WT male mice of BMSC, under the action of osteogenesis, into fat revulsant observatio n of BMSC differentiation capacity changes.At the same time testing related osteoge nesis, into fat gene expression.For in vivo experiments, we male mice to CMKLR1- KO and WT source of castrated surgery to remove the testicles, man-made osteo porosis model, to explore, under the condition of pathogenesis of osteoporosis in mi ce, CMKLR1 absence or not on bone metabolism regulation gene.Results:1. CMKLR1 knockout mice under physiological conditions and CT bone loss, H&E staining found seriously reduce the trabecular bone, bone trabecular thickness.And serum testosterone levels decreased significantly;2. CMKLR1 knockout mice so urces of differentiation of BMSC in into fat, bone morphogenic process significantl y suppressed.And the cut related marker gene expression.Conclusion:1. CMKLR1 gene knockout can inhibit the bone marrow mesenchymal stem ce lls(BMSC) between the osteogenetic differentiation, and can be cut through Runx2,ALP maker genes associated with osteogenetic differentiation.And CMKLR1 gene is missing, chemerin/CMKLR1 signaling pathway is restrained, also interfere with ge ne expression of adiponectin, Fabp4 and into fat of BMSC differentiation has playe d a certain inhibition.2. CMKLR1 gene in physiological conditions, the lack of mal e mice bone loss is evident, however, when we in mice castrated surgery and treat ment of osteoporosis caused by pathological condition, type found WT mice bone l oss obviously, is given to illustrate the success of the building, the type and KO m ice in osteoporosis has no obvious differences on the basis of change.3. Under phy siological conditions, male mice CMKLR1 gene deletion can lead to significantly lo wer levels of serum testosterone and its receptors, suggesting that CMKLR1 knocko ut will affect reproductive secrete testosterone, which directly affect the bone metab olism.