The Effection Of Yiqi Huo Xue Recipe To T He Expressing Of Sensitive Factors Associa Ted With Radiation Induced Lung Injury

To analyze the function of Yiqi Huoxue Recipe(gavage the irr adiated rats)in radiation induced lung injury, and then Elaborate it w ith three methods(RT-PCR、 Immuno histochemistry), observe mRNA and protein expression variation of TGF-β1、 TNF-a、 NOS3 and MTHFR in the tissue of radiation-induced lung injury, to analysis the particular mechanism of the injury from protein and gene levels.108 healthy adult rats were equally divided into three groups randomly: named separately as simple radiation group(model group, n=36), radiation and Chinese herbology group(Chinese herbology gro up, n=36), and blank control group(control group, n=36). Confirm th e radiation region under x ray-Simulation Localization System: the up border to the top of the right lung, the lower bound for the right-h and Diaphragm top, the left border is right-hand edge of the spinal c ord, right up to the right side of the thorax outer boundary. Radiatio n field is generally 3 × 3cm size. Occlusion of the left lung and me diastinal lead weights, totally 30 Gy, 5Gy / time, a total of six times.Rats of Model group and Chinese herbology group were irradiated with 6MV-X Linear Accelerator,control group rats just anesthesia, bu t not radiation.Before irradiation,the rats were anesthesiaed with 7%chloral hydrate aldehyde(5?kg-1) by intraperitoneal injection, then pro ne on the platform and fixed them(nail four nails on the plank, the l imbs of anesthetized rats fixed on the nail were rubber band). From the first day of radiation,rats of Model group and Chinese herbology group were fed with Yiqihuoxue prescription,1ml respectively, 2 times a day,at 08:00 and 20:00. Six rats were randomly selected at the end of 2,4,8,12,16,20 weeks, open the chests of these rats i mmediately right after intraperitoneal anesthesiaed, take the right lung tissue and save in right way. PCR methods were used to detect dyn amic changes of TGF-β1, TNF-a, NOS3, MTHFR mRNA expression at each time pointand immune histochemistry were used to detect the dynamic changes of TGF-β1, TNF-a, NOS3, MTHFR protein, and t he experimental datas were statistical analysised with SPSS.17.0.RT-PCR and immunohistochemistry results suggest that TGF-β1, TNF-a, NOS3, MTHFR mRNA and protein expression after irradiatio n among the 2th-20 th weekends appear a first increaseing and then do wnward trend. Their protein and mRNA expression began to increase from the 2th weekend, 8th weekend to the peak expression, decrease d from 12 th weekend. MRNA and protein’s expression trends of Chi nese herbologygroup were similar with the model group, but there ar e different variations at different time points.We found that TGF-β1, TNF-a, NOS3 and MTHFR genes were sensitive factors when radiation-induced lung injury occurs. Yiqihuoxu e can regulate the expression of TGF-β1, TNF-a, NOS3, MTHFR fro m transcription and translation level in radiation-induced lung injury at different time points. This may be the molecular mechanisms of d rug prevention and treatment in radiation-induced lung injury. After t he end of radiotherapy,relevant cytokines’ expression continued to 16 weekend, it suggested that the treatment time of radiation-induced lu ng injury should be extended to 2-3 months after the end of radiati on. And at different times, the priorities and the rules of treatment is different to be emphasesed.