The Preparation Of Porous Antibacterial Bone Repair Materials HAPw/NmZnO Technology And Animal Experimental Study

[Objectives] To manufacture powder hydroxyapatite nanometer zinc oxide whisker HAPw/nmZnO antibacterial bone repair materials into porous granular; To discuss intra-individual biological performance of porous granular HAPw/nmZnO antibacterial bone repair materials by animal experiments.[Materials and Methods] Using HAPw/nmZnO composite as raw material, add the silicasol calcined at different temperatures and X-ray diffraction (XRD) analysis to determine the silicon sol and HAPw/nmZnO reaction temperature. Then, control grain size in the 100—250 microns of pore-forming agent polymethyl methacrylate (PMMA) and 30 wt% of silica sol as binder, with konjac also unwilling chitosan (KGM) gel after cold forming sintering of crushing sieving, and application of X-ray diffraction (XRD), scanning electron microscope, universal testing machine, Archimedes drainage method of test and analysis. Choose 15 healthy tree shrews, male and female unlimited, each back muscles were implanted into four artificial bone material, namely porous granular HAPw/nmZnO antibacterial BIOS bone bone repair materials, Switzerland, France ATLANTIK artificial bone, domestic Kim spirit of artificial bone, bone graft HAPw/nmZnO antibacterial bone repair materials as the experimental group, the rest three groups are treated as the parallel control groups. Randomly choose 4 animals to death each time after 4,8,12 weeks, remove muscle bag implanted artificial bone material, and generally observe the shape of the residual material, histopathological observation, determination of alkaline phosphatase activity and calcium content in order to investigate porous HAPw/nmZnO antibacterial biological properties of bone repair materials.[Results] In the first experiment, XRD spectrum along with the change of calcining temperature, calcining temperature over 600 ℃ when the silica diffraction peak disappear, and the calcium silicate zinc silicate impurity peak, silicon dioxide and HAPw/nmZnO antibacterial biological properties of bone repair materials chemical reaction happened. It also indicates when add silica sol as binder sintered porous HAPw/nmZnO composites, calcination temperature should not exceed 600 ℃. Sinter porosity is the most ideal two hours 600 ℃ heat preservation; Pore-forming agent content is 40 vol% porosity is 64.2%, the compression strength is 1.1 MPa. SEM results show HAPw/nmZnO after preparation into cellular morphology of nmZnO had no obvious effect, porous composite material has three holes, one kind is formed by pyrolysis pore-forming agent and macro big hole, another kind is by konjac glucan-mannan glycan ready-made macro pores after pyrolysis, then a is a composite material between micro holes formed from the accumulation of each other, the latter two holes will macro big hole connected. In the second experiment, HE staining showed the experimental group and control group muscle interstitial are visible within a small amount of calcifications (about 5%), implant materials surrounding fibrous tissue hyperplasia, no obvious inflammation cells are linked together. Masson staining showed the experimental group and control group implant materials are seen around a small green dye collagen fiber; Alkaline phosphatase activity and calcium content determination of the experimental group with domestic Kim Swiss Bio-Oss spirit set of bone graft and bone meal group difference was statistically significant, the domestic Kim Swiss Bio-Oss spirit set of bone graft and bone meal group is better than that of the experimental group, there was no statistically significant difference with France ATLANTIK artificial bone group.[Conclusion] Applying PMMA as pore-forming agent, using the method of KGM gel cold-press sintering molding after crushing sieving could manufacture irregular porous particle diameter distribution between 100—200 microns, porosity is less than 64% of porous HAPw/nmZnO antibacterial bone repair materials.Porous granular HAPw/nmZnO antibacterial bone repair materials, Switzerland, France ATLANTIK artificial bone, domestic Kim spirit artificial bone graft in bone implant tree shrews back muscles are not ectopic ossification after 12 weeks, but all showed good biological activity.