Molecular Characterization And Preclinic Investigation Of Anti-endotoin Peptides From Bombina Maxima Under Environmental Stress

Infection is regarded as one of the most important reasons which cause human death. Although many clinic drugs could efficiently kill the bacteria, there was no efficient treating method to deal with septicopyemia caused by endotoxin after infection. Endotoxin is an important outer membrane component of Gram-negative bacteria, which induce a serials cascade effects once entering into blood or liberating abundantly, causing multiple organ dysfunction, decline of immunity and eventually patients death. Currently, clinic adopted methods to treat endotoxin-induced septic shock will mainly relay on glucocorticoid to protect the organ functions, countermeasures directly against the endotoxin are lacking. Bombina maxiam is a specific amphibian distributed only in south-west of China all over the world. The living environments of this species are very bad and easily stimulated with bacteria, ultraviolet radiation, which make the skin secretions of this species rich of bio-active components and provide valuable investigation targets. Present application using Bombina maxiam as research template, characterize the specific component which having endotoxin neutralizing activity from skin secretions after stimulated by bacterial endotoxins and reveal the possible mechanisms of the molecule to exert the endotoxin neutralizing activity. Carrying out this research has both scientific and practical importance. It might provide novel ideas and clues to treat the septicopyemia and septic shock.