The Biological Characteristics Of EGF Induce HCT-116 Colon Adenocarcinoma Cell Neuroendocrine Differentiation

Objective:Epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) inhibitor cetuximab induce and block to induce HCT-116 colon adenocarcinoma cell neuroendocrine differentiation (NED), explore the changes and possible related factors in inducing and blocking induce HCT-116 colon adenocarcinoma cell of proliferation, apoptosis and invasion abilities.Methods:Cultured HCT-116 colon adenocarcinoma cells on 5th day,21th day and 28th day, immunohistochemical stain and observed the expression of CgA, Syn, Ki-67, Bcl-2, CEA, CA19-9 and CDX2 in before and after culture HCT-116 colon adenocarcinoma cells; MTT draw growth curve of HCT-116 colon adenocarcinoma cells; flow cytometry (FCM) detect the cell cycle distribution of HCT-116 colon adenocarcinoma cells; Transwell chamber detect invasion ability of HCT-116 colon adenocarcinoma cells.Results:Cultured HCT-116 colon adenocarcinoma cells with EGF, leading to increase Ki-67, Bcl-2 and CgA expression, increase cell proliferation activity, reduce the number of apoptosis, increase the cells proportion of S. G2 phase, increase the number of cell thread Matrigel. Comparing with regular culture, adding alone cetuximab culture and adding cetuximab and EGF, the difference is statistically significant (P<0.05).The expression of CDX2、CA19-9、 CEA have no statistically significant (P> 0.05) in each group.Conclusion:(1) EGF can induce HCT-116 colon adenocarcinoma cells neuroendocrine differentiation (NED), and increase cell proliferation,decrease the capacity of apoptosis.increase cells proportion of S, G2 phase and cell invasiveness; (2) cetuximab inhibits EGF-induced HCT-116 colon adenocarcinoma cell NED. and inhibit EGF-induced HCT-116 colon adenocarcinoma cells proliferation, promote apoptosis, reduce the proportion of cells in S,G2 phase and decrease cell invasion ability.cetuximab have no influence on NED, cell proliferation, apoptosis to conventional culture HCT-116 colon adenocarcinoma cells.(3) CDX2, CA19-9, CEA are not involved in the regulation of NED in HCT-116 colon adenocarcinoma cells.