The Effect To Intervertebral Disc Degeneration And Corresponding Molecular Mechanisms Of Naringin

Objective:The aim of the present study was to determine the potential anti-inflammatory effect of naringin in Interleukin-1β induced rat nucleus pulposus cells and its corresponding molecular mechanisms.Methods:We also assessed the effects of naringin on the expression of MMP-1, MMP-3, and MMP-13on mRNA and protein levels, detecting by real-time reverse transcription-polymerase chain reaction analysis, and by western blotting or enzyme-linked immunosorbent assay (ELISA) separately. Nuclear factor kappa B (NF-κB) and inhibitor of kappa B kinase a (IκBα) in cells also determined by western blotting, and the involvement of signal pathway was assessed by transient transfection and luciferase activity assay.Results:As a result, we found that treatment of IL-1β-induced nucleus pulposus cells with various concentrations of naringin caused a potent decrease of MMP-1, MMP-3, and MMP-13. In addition, naringin treatment of cells also inhibited the activation of NF-κB and enhanced the expression of IκB-α.Conclusions:In view of the results, we provide evidence that naringin can protect nucleus pulposus cells by attenuation the activity of MMPs through NF-κB signaling pathway. As a conclusion, naringin could be further developed and used as a component for prevention and treatment of disc degeneration.