| Objective:The issue with traditional Chinese medicine theory,combined with modern experiments, established rat model of endometrial hyperplasia, intervent with Chinese medicine of replenishing kidney and health care spleen, By observing the rat uterus Chinese medicine of replenishing kidney and health care spleen endometrial hyperplasia topically active substance VEGF, BAX, BCL-2 levels of influence,Influence of traditional Chinese medicine for kidney and spleen uterine microenvironment, Then discusses adolescent medicine treatment of kidney and spleen DUB mechanism of action, provide more open treatment ideas for the clinical treatment of adolescent dysfunctional uterine bleeding.Methods:In this experiment, juvenile female SD rats as the research object, estrogen stimulation of endometrial hyperplasia model established after ovariectomy to estradiol valerate, Chinese medicine of replenishing kidney and health care spleen, medroxyprogesterone acetate tablets and granules Gongxueting experimental medication. The 90 underage female rats at 30 days of age, body weight 90+lOg OK ovary removal surgery, in which 15 rats were sham-operated group only switch abdominal surgery, three days after the injection of penicillin to prevent infection, painted five consecutive days rat vaginal exfoliated cells, keratinocytes that no surgery was successful. Successful surgery the next day in rats fed a total of 45 days of estradiol valerate, endometrial pathology prompted hyperplasia is the successful model in rats. The successful model rats were randomly divided into five groups, namely, model group, western medicine group, Gongxueting group, Chinese medicine of replenishing kidney and health care spleen high and low dose groups of 15.Results:(1) the thickness of the experimental group were significantly thinner than the model group, increased the number of glands.(2) Chinese medicine of replenishing kidney and health care spleen can the endometrium was significantly increased VEGF expression (P <0.05, and P<0.01);(3) Chinese medicine of replenishing kidney and health care spleen can the endometrium BAX expression was significantly increased (P <0.05), BCL-2 expression was significantly lower (P<0.05), BAX/BCL-2 ratio was significantly decreased (P<0.05,and P<0.01).Conclusion:The experiment proved that Chinese medicine of replenishing kidney and health care spleen can only increase the expression of VEGF in Uterine to achieve the restoration of endometrial blood vessels, complete hemostasis purposes; by increasing the ratio of BAX/BCL-2 and induce apoptosis in abnormal proliferation of the endometrium, to alter endometrial hyperplasia pathological state. Tip Chinese medicine of replenishing kidney and health care spleen regulate uterine activity factor of the role of local, oriented only to improve the mechanism of the local microenvironment provides an experimental basis for the clinical use of Chinese medicine of replenishing kidney and health care spleen punch Treatment puberty anovulatory DUB offers research and theoretical support. |
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