The Clinical Efficacy Observation Of Lung Tumor Inhibition Cream Combined With DC-CIK Cells On Advanced Lung Adenocarcinoma And The Exploration Of The Corresponding Mechanism In Cell Apoptosis

Objective: The present research was aimed to observe the clinical efficacy of Lung tumor Inhibition cream combined with DC-CIK cells,and try to explain the potential mechanism of the combination acting on tumor cell apoptosis.AS a result,a new possible theoretical basis for the combination in the treatment of advanced lung adenocarcinoma, and a new combined treatment of traditional Chinese and Western Medicine of lung cancer was provided.Methods: 54 patients with advanced lung cancer patients enrolled were randomly divided into Immunotherapy group and Chinese herbs combined with immunotherapy group, each group have 27 cases. After 4 months of treatment, observing the main clinical symptoms,tumor size, quality of life, immune parameters, serum sFas, sFasL concentration changes and adverse transfusion reactions in patients after given DC-CIK cells were observed.Results:(1) Changes in clinical main symptoms: two groups in improving symptoms such as cough, chest pain, hemoptysis, poor appetite, dry mouth showed differences.(P <0.05) and traditional Chinese medicine combined with immunotherapy group was superior to the immunotherapy cells group; in improving fatigue and shortness of breath,there was no significant difference(P> 0.05).(2) Changes in tumor size: The two groups differred in easing lesions(P <0.05), traditional Chinese medicine combined with cell immunotherapy was superior to simple cell immunotherapy, in stabilizing lesions, the two groups had no significant difference(P> 0.05).(3) Changes in the quality of life: there were differences between the two treatment groups(P <0.05), traditional Chinese medicine combined with immunotherapy group was better than the simple cell immunotherapy group(4) Changes in immune parameters: the two treatment groups had difference in increasing CD4+, decreasing CD8 + and increasing the ratio(P <0.05), traditional Chinese medicine combined with immunotherapy group was better than the simple cell immunotherapy group; As for CD3+,there was no significant difference(P> 0.05).(5) Serum sFas, sFasL concentration changes: the serum sFas, sFasL concentration had no difference between different sex and age(P> 0.05). the serum sFas had difference in different stages(P <0.05), while sFasL had no difference(P> 0.05);the two groups in lowering serum sFas, sFasL concentrations were different(P <0.05), traditional Chinese medicine combined with immunotherapy group was better than the simple cell immunotherapy group.(6) Safety evaluation: By observation,only some patients fevered, there were not peripheral blood cells decreasing, significantly gastrointestinal discomfort, liver and kidney function impairment and adverse allergic reactions, rash, edema in the two groups.Conclusion: Lung tumor inhibition cream combined with DC-CIK cell therapy compared with simple DC-CIK cell therapy could be more effective in improving clinical symptoms and life quality of patients with primary and reducing solid tumors volume, improving immune function, reducing the incidence of fever and other adverse reactions, while decreasing the concentration of serum sFas, sFasL,possibly promoting apoptosis of tumor cells by this way.