Prepatation And Evaluation Of Shendan Quzhen Spray

Objective:To proparate and evaluate the Traditional Chinese Medicine named shendan quzhen spray used to improve eczema, and then, study on its preparation process, quality standard. Methods:The extraction concentration and forming process were optimized by single factor test and orthogonal test, in order to determine the preparation process, used mat.rine, oxymatrinc, paeonol, momordin Ic as auxiliary indexes; With thin layer chromatography (TLC) each other to medicine by TLC, and the HPLC analytical method has been developed for the assay of some medicine drug in this prescription, formulating optimal quality standards; Investigate the stability of the preparation at room temperature and heating speed up conditions. Results:The optional preparation process of shendan quzhen spray:take wild chrysanthemum flower, cortex moutan. Add 10 times the amount of the water vapor distillation,1 hour each time. Received distilled liquid 250ml, filtcrthe medicine residue collecting extracted liquid, reserved the distillate and extraction liquid. Take sophora flavesccns Ait, plus 0.1% Hcl acid solution, soaking 3 times with 60℃ each time 10 timess、8times.6timcs,1 hours each time. Combine the extract added 5% NaOH solution adjust pH to 5-6, concentrated to 500 ml, adjusted pH to 10～11 within 5% NaOH solution, adding sodium chloride 50g, low temperature for 12 hours, Fil tratc, precipitation with 0.1%IfCl aqueous solution soaks for 1 hour every time.10 t.imeseach time. Reserved Liquid water. Take fructus Kochiae, cortex Pseudolaricis, decocted 3 times, each time within 10 times、8times、6times the amount of water,1.5 hours every time; The filtrate was collected, mixture with the extract of wild chrysanthemum flower and Cortex Moutan, concentrated to 60℃ when the relative density is 1.10-1.15, adding alcohol to make the alcohol content, to 70%, standing for 12 hours, filtering, recovering alcohol, The quantity of join Twain-80 into solution is 20g. mix With the distillate of wild chrysanthemum and Cortex Moutan, then add the acid extract, of Sophora flavescens, add water to 1000ml,2 hours of high speed homogenate, 10g number of citric acid adjust pll to 5,5-6.5, adding sodium benzoate amount, With thin layer chromatography view, the test of menthol, Cortex moutan, wild chrysanthemum and control substances are the same color spots in the same location, according to determination of spray content, determinations of Matrine and Oxymatrine are 2.2mg.ml at least. Conclusion:the preparation process of Shendan quzhen spray is stable and clear, quality control method is stable feasible and effective and the reproducibility is good.