The Study Of The Protection Of Nicotine Against Acute Pancreatitis

This object is via detecting the levels of TNF-α、IL-10、IL-12 in the serum, the differentiation of DCs and pathological changes of pancreatic tissue in the AP models. It aimed to investigate below aspects:To investigate a certain range of concentrations of nicotine can significantly reduce the acute inflammatory reaction.To investigate a certain range of concentrations of nicotine can decrease the mortality rate of AP mice.To investigate nicotine regulate the immune status of AP may be via inducing the differentiation of DCs to CD11clowCD45 RBhigh DCs.The 100 BALB/c mice were separated into five groups as follows: control group, SAP group, SAP with nicotine(0.1mg/kg, 0.2 mg/kg, 0.3 mg/kg)treatment groups. Alternatively, intraperitoneal administration of 2 doses of L-arginine(40 mg/kg) at hourly intervals was used to induce AP, the control was administrated with 0.5 ml normal saline. Then, the mice were sacrificed after the AP 0, 12, 24, 48, and 96 hours respectively, with four animals at each time point, the pancreatic tissue was kept to pathological examination. CD11clowCD45 RBhigh DCs were isolated by Magnetic microbeads from mice. CD40、CD80、CD86 and MHC-II were analyzed with Flow cytometry, and the cytokine levels of IL-10、IL-12 and TNF-αwere measured by ELISA. Another 96 mice were employed to evaluate the effect of Nicotine on the mortality rate following SAP respectively. The mortality was observed at each time, the observation period was 8 days.Treatment with nicotine could significantly increase the amount of CD11clowCD45 RBhigh DCs and was showed in a dose-dependent manner.However, nicotine could significantly decreases the expression of CD40、CD80、 CD86 as well as MHC-II. Compared with AP, the treatment ofnicotine could decrease obviously the levels of cytokine of IL-12 and TNF-α at each time point, in contrast, the level of IL-10 was elevated significantly. Beyond that, the administration of nicotine could reduce obviously the pancreatic injury and was showed in a dose-dependent manner. The AP mice treated with nicotine(0.3 mg/kg) at 12h、24h、48h、96h was found that the survival rate was slightly increased. In contrast,given nicotine(0.1 and 0.2 mg/kg) can notably elevated the mortality rates.Within a certain concentration range, nicotine can significantly reduce the inflammatory reaction of AP and pancreatic tissue damage, which also can improve the survival rate of AP. At the same time, the amount of DCs was significantly increased. Thereby nicotine may improve the prognosis of AP by inducing the differentiation of DCs.