Lipid-lowering Mechanism Of Physalis Leaf Tea In Hyperlipidemic Rats

[Objective]To investigate the lipid-lowering effects of Physalis leaf tea on high fat diet fed rats, observed related organizations morphological changes, and preliminary inquiry lipid-lowering mechanism of Physalis leaf tea from molecular and protein levels.[Methods]Choose 40 male SD rats, when the rats after adapt to feed, randomly selected 8 rats as control group A,the remaining 32 rats were fed high-fat diet. 35 days later, blood test the level of their TC、 TG, compared with control group to confirm the success of high-fat model. After the success of modeling, the rats based on TC and TG levels were randomly divided into 4 groups: Hyperlipidemia model group B, the positive control group C, Physalis leaf tea low dose group D, Physalis leaf tea high dose group E. Record daily food and water intake, measured body weight every three days. After 35 days, observe experimental groups rate’s organ index,serum TC、TG、HDLC、LDLC level, observed the tissues morphology of liver, kidney, spleen and heart with HE stain. Extract total RNA of rat liver and adipose tissue, observed the mRNA expressins of LDLR, HMG-CoA, CYP7A1, PPAR in liver, HSL in adipose tissue with real-time PCR. Extract total protein of rat liver, observed the liver CYP7A1 protein expression with Western Blot.[Results] 1、Physalis leaf tea can reduce the body weight of rats with hyperlipidemia, reduce liver and fat index, lower TC, TG, LDLC lever, increased HDLC levels.2、Physalis leaf tea can repair hyperlipidemia rats liver and kidney tissue morphology that caused by high fat die, such as liver cell boundaries become clear, relieve hepatic steatosis, hepatocellular cord become normal, lobular structure tends to complete. While the kidney tissue hypertrophy glomerular volume have different degrees of smaller, the degree of glomerular basement membrane thickening was significantly reduced, as well as significantly improved the rat kidney pathological changes. So it means Physalis leaf tea can improve the results of pathological changes in kidney and liver tissue, and promote liver tissue lipid and cholesterol metabolism, lower blood lipids.3、Compared with the model group, the hyperlipidemic rats that intragastric Physalis leaf tea mRNA expressions of LDLR、CYP7A1、PPAR in liver, HSL in adipose tissue had significantly or very significantly increased, the mRNA expressins of HMG-CoA in liver had significantly lower. Suggesting that Physalis leaf tea through comprehensive regulation of these genes to achieve the effect of lowering blood lipids.4、Compared with the model group, the hyperlipidemic rats that intragastric Physalis leaf tea protein expression of CYP7A1 in liver had increased, the positive control group、Physalis leaf tea low dose group and high dose group were increased the expression level 84%、37%、81%,suggesting that Physalis leaf tea can increase the expression of CYP7A1 in rat liver tissue in translation level.[Conclusion]Physalis leaf tea have a good lipid-lowering effect on high fat diet-induced hyperlipidemia rats, its possible lipid-lowering mechanism: ①reduce hyperlipidemia rats body weight, reduce serum lipid level of hyperlipidemia rat, improve rat liver damage and adipose tissue accumulatin, increased fat tissue metabolism; ② improve hyperlipidemia rats liver and kidney tissue pathological changes, mitigation the damage of liver and kidney tissue, promote liver tissue lipid and cholesterol metabolism;③ increase serum HDLC level, decreased serum LDLC level, increased fatty acid metabolism; accelerate the rate of cholesterol into bile acids, increase fecal bile acid discharge quantity; promote lipolysis、influence fat metabolism ,aim to reduce blood lipids;④ affect lipid-associated protein CYP7A1 expression at the translational level, promote bile metabolism, lower blood lipds.