The Expression Of Antimicrobial Peptide LL-37 In The Bladder Urinary Tract Carcinoma

ObjectiveExpression of endogenous antimicrobial peptide LL-37 in urine and bladder cancer tissues of patients with healthy physical examination and bladder cancer tissues,The levels of LL-37 in the supernatant of bladder cancer cell line (EJ, T24) and normal urinary tract epithelial cell line (SV) were observed by bacterial constituents from the cell level of LPS stimulated by bacterial constituents,to investigate the relationship between endogenous antimicrobial peptide LL-37 and bladder urinary tract epithelial carcinoma. Methods1.Methods 30 patients with bladder urinary tract cancer and 30 sex and age were selected to collect the fresh morning urine of the subjects, and the levels of LL-37 in urine were detected by ELISA.2.Methods 30 cases of bladder cancer patients were selected for surgical resection of tumor tissue specimen and tumor tissue base and surrounding tissue specimens, and immunohistochemistry method was detected to express LL-37 expression in tumor tissue.3Methods 30 cases of bladder cancer patients were selected for surgical resection of tumor tissue specimens and tumor tissue samples and tissue specimens. RT-PCR method was detected to detect the expression of LL-37 in tumor tissues.4.Cultured human bladder tumor cell line (T24,EJ) and urinary epithelial cell lines (SV), using different concentrations of LPS stimulated cells. ELISA method for detection of T24, EJ, SV cells before and after LPS stimulation by culture supernatant of LL-37. Results 1.ELISA results showed that healthy volunteers, in the urine of patients with bladder urothelial cancer levels of LL-37 were 2.02+0.06ng/ml and 24.32+ 0.02ng/ml. LL-37 levels in the urine of patients with bladder urothelial carcinoma significantly increased (P< 0.001).1.Immunohistochemistry results showed that LL-37 was mainly expressed in the inflammatory cells, including macrophages, neutrophils and lymphocytes in the cell membrane and cytoplasm, cancer surrounding tissue, smooth muscle and vascular wall, a brown yellow granular change. The positive expression of the cancer peripheral group was negative for the control tumor cells.2.Reverse transcription-PCR results showed that PCR product of actin as a positive control, bladder cancer group LL-37 expression is not obvious, and bladder cancer group compared to expression of LL-37 in cancer surrounding tissue increased. Quantitative PCR Real-time showed that the expression of LL-37 in the surrounding tissues was about 30 times higher than that in bladder cancer tissues LL-37.3.ELISA results showed that normal urothelium cells in LPS stimulated cell culture supernatant levels of LL-37 increased significantly, bladder tumor T24 cells in LPS stimulated cell culture supernatant levels of LL-37 increased significantly, EJ Cells in LPS stimulated cell culture supernatant of LL-37 levels did not increase significantly.ConclusionsTo adapt to the growth of bladder tumor micro environment exist in a certain amount of LL-37, LL-37 may play a role in the pathogenesis of bladder urothelial carcinoma, whether a bladder tumor has led to LL-37 or LL-37 induced tumorigenesis, still need further research and discussion.