Application And Effects Of Low-intensity Laser Repair Degenerative Disc Disease In The Pig Model For The Expression Of Inflammatory Cytokines

Objective:To observe the degenerative disc disease of the animal model of Bama pig and investigate the effect of low-energy laser disc degeneration; the expression inflammatory factors in dise, as the low-energy laser in the treatment of disc herniation provided better theoretical basisMethods:Bama miniature pigs 9, boar five, four sows were randomly divided into three groups, each group 3, respectively, the control group(Con group), blank model group(Mod group) and low-power laser therapy group(Las group). Con group without any treatment, observed two months, Mod team used 16 G needle into pig L2-3, L3-4, L4-5,L5-6 disc destruction, and then observe a month, Las group set up After the pig disc degeneration model, feeding a month and then the disc has been processed low-energy laser therapy, and then observed a month. For pre-treatment and post-treatment MRI scans a month to verify the success of the modeling and treatment. After his execution Bama miniature pig pig remove the complete disc(including the lower thoracic and lumbar spine), was visually observed and generally take a photo, remove the disc intervention and control groups, the line HE staining to detect the morphological changes. Using SPSS 19.0 software for statistical analysis, experimental data wereexpressed as mean ± standard deviation(± s) to that the groups using ANOVA, repeated measures analysis of variance within the group, the correlation between two variables Pearson correlation analysis to P <0.05 was considered statistically significant.Results:1. Imaging observation: the intervention of the three groups of pigs had intervertebral disc MR detection, Con pigs disc T2-weighted images showed high signal,the signal even nucleus, bright, clear boundary between the nucleus and annulus, highly consistent between the disc; Mod group nucleus pulposus decreased signal intensity on T2-weighted, disc signal to a dark gray or black low signal intensity, and gradually narrowed disc space; Las treated in T2-weighted images showed high signal intensity,disc height declined marrow Nuclear signaling a more uniform, the nucleus pulposus and annulus border appears partially obscured.2. General observation of samples: Eye View of Con group nucleus pulposus white jelly, rich in water inside the nucleus, annulus structure neatly arranged in concentric circles; Mod group puncture site disc thickness decreased, dark nucleus, content reduced annulus structure rough; Las group disc thickness decreased, the nucleus of local color gray hair, the nucleus was reduced.3. HE staining Results: Con annulus structured group, the nucleus pulposus and annulus clear boundaries, arranged in a grid-like structure of collagen rules; Mod annulus disorder group, the nucleus matrix disintegrates almost no nucleus cells.Degenerative intervertebral disc disorder and even rupture, the nucleus reduced,reducing the number of cells, moisture reduction, and inflammatory cell infiltration; Las group within intervertebral disc structural disorder, endplate cell level is unclear,disorganized, there are a few inflammatory cell infiltration.4.The inflammation immunohistochemistry results Media: disc TNF-α, IL-1content changes: Compared with Con group, Mod group TNF-α, IL-1 levels were significantly increased, the difference was statistically significant( P <0.05). Comparedwith the Mod group, Las group TNF-α, IL-1 levels were significantly lower, the difference was statistically significant(P <0.05).Conclusion:1. Low energy laser therapy on the damaged disc Bama miniature pigs have some repair;2. Low-energy laser to Bama miniature pigs damaged disc protection and repair mechanism may inhibit the inflammatory response.