The Effect Of Triptolide In Multiple Myeloma Cell Line KM3Cells And Its Mechanism

Objective To investigate the effect of triptolide on the proliferation, apoptosis and and H3K4me3protein expression in multiple myeloma cell line KM3cells.Methods The MM KM3cell line was cultured with triptolide. Apoptosis was evaluated by Annexin-V-FITC/PI-labeled flow cytometry; Cell proliferation was detected by MTT method The expression of H3K4me3in KM3cells was assayed by Western blotting and confocal microscopy.Results Triptolide had obvious inhibition on proliferation of KM3cells, showed a dose and time dependence, the50%inhibiting concentration (IC50) was respectively (104.97±0.16) nmol·L-1.(77.11±0.05) nmol·L-1and (38.95±0.17) nmol·L-1after24hour.48hour and72hour treatment with triptolide. Triptolide had obvious inhibition on apoptosis of KM3cells, and with the increase of triptolide concentration, the apoptosis ratio increased gradually. Respectively40nmol·L-1,80nmol·L-1,160nmol·L-1concentrations of triptolide intervention48h. The early apoptotic rates were (39.98±2.76)%,(43.89±1.82)%and (47.12±2.03)%, the total apoptosis rates were (48.97±1.78)%,(53.72±2.21)%and (60.75±2.43)%. Western blot results showed that the expression of H3K4me3protein in KM3cells decreased gradually with the increased of the concentration of triptolide. Confocal laser scanning microscopy showed H3K4me3accumulated in the nucleus, after48h intervention of80nmol·L-1triptolide, the mean value of fluorescence intensity of H3K4me3in KM3cells (21.96+0.34) was significantly lower than that in the control group (39.86±0.47)(P<0.05).Conclusions Triptolide inhibited cell proliferation, induced cell apoptosis, apreliminary study found that by H3K4me3related protein pathways to induce apoptosis..