Extraction And Pharmacological Study Of Plant Polysaccharides In The Nettle

Polysaccharides are natural macromolecules, constitute an important component of all living organisms to maintain a variety of physiological functions of the body are interrelated. After years of research found that polysaccharides have anti-tumor, anti-inflammatory, antiviral, hypoglycemic, anti-aging, anti-radiation, anti-clotting, blood sugar and other bioactive lipids.Nettle of angiosperm, nettle (Urticaceae), Urtica (Urtica.L.), Is a plant nettle Urtica fissa E.Pritz. Of dry grass, which are annual or perennial wild herbs, nettle natural growth in the hillside, next to the road, in the bushes and forests. This cheap medicinal polysaccharides resources in anti-tumor, anti-oxidation relatively uncommon research has important significance and value of their research pharmacological action.In this issue, we select plants nettle (Urtica fissa E.Pritz) for the study, made the following specific research areas:By water extraction and alcohol precipitation total polysaccharides Guangxi nettle extract and determine the content of polysaccharides in which glucose standard curve. Method for Extracting preclude the accelerated release by heat treatment prior to heating the extraction, the first meal of petroleum ether reflux medicines to remove part of the fat-soluble fraction of herbs meal degreased using water extraction and alcohol precipitation method of extraction, use orthogonal experiments to determine the optimum conditions nettle plants polysaccharide is A1B2C1D3, namely soaking2h, reflux2times to extract2h, a temperature of85℃. Then use seavge method of eliminating protein, H202dialysis to remove oxide pigments, the addition to the polysaccharide protein, pigment after95%ethanol, ethanol, acetone, diethyl ether, respectively, to give fine nettle and a polysaccharide component. The purified polysaccharide in vivo anti-tumor, anti-oxidation, anti-oxidation in vitro.The effects of:(1) inhibition of nettle polysaccharide on H22tumor bearing solid tumors.(2)for H22mice thymus Index, spleen index, glutathione (GSH) activity of nettle polysaccharides, catalase (CAT) Activity of malondialdehyde (MDA) in effect.(3) Nettle Polysaccharide hydroxyl radicals, superoxide anion radical activity capacity.Experiment with nettle of the extracted polysaccharide component of the qualitative and quantitative analysis, the results show that we have to extract the crystal is nettle and a polysaccharide component, and its content was98.22mg/g, the yield was1.56%, purity of62.61%. Thus, we roughly estimate what the quality of the original ingredients to be extracted, large-scale extraction and purification for subsequent pharmacodynamic studies sufficient time to prepare. It was observed that nettle plant polysaccharides in high, medium and low dose group could significantly inhibit tumor growth in mice, inhibition rate was33.13%, respectively,29.43%,13.78%, and the results were statistically significant (P<0.05); nettle in different degrees polysaccharide thymus and spleen index of tumor-bearing mice, significantly improving the quality of survival of tumor-bearing mice. In vivo anti-tumor experiments, high, medium and low dose group group mice serum SOD, GSH, CAT activity than the control group, while MDA content is lower than the normal group, the results were statistically significant (P<0.05) guess nettle polysaccharide antitumor effect may be related to enhanced antioxidant capacity, inhibition of lipid peroxidation.Through in vitro nettle polysaccharides on O2-scavenging effect on scavenging effect of·OH, prima facie nettle polysaccharide has some antioxidant effects. Polysaccharide extract of nettle different antioxidant systems exhibited certain antioxidant activity. Its various systems, their antioxidant capacity were weaker than reference standards. Overall, nettle polysaccharide has a hydroxyl radical scavenging ability and superoxide anion.