The Study On The Extraction Of Effective Components Of Herbal By Bacillius Subtilis

Chinese medicine refers to in the theoretical system of traditional Chinesemedicine under the guidance of a clinical medication, is endemic to China, a kind ofdifferent from the natural medicine of drug, although herbal materials in naturalanimals, plants and minerals, but the treatment mechanism and method are andWestern medicine recognized natural medicine has a essential difference. Payattention to the traditional Chinese medicine in the treatment of diseases is the overall,medication but also pay attention to multi targets comprehensive treatment, so thetreatment of Chinese medicine is usually a mixture. At present, there are12807varieties of Chinese herbal medicines in statistics, including87%plant sources ofmedicinal plants. Its medicinal active ingredients were wrapped in the lignin andcompact structure in. Traditional Chinese medicine processing method for physicalmethods, mainly grinding, baking and cooking and so on, its purpose is destruction ofcells of plant cell walls and intercellular matter connections. Some processingmethods can change the drug resistance, reduce the side effects. Although thesemethods of inheritance for thousands of years, clinical confirmed its validity. But theprocess is complicated and the uncertainty makes it difficult to meet the demand fordrugs.In this paper the microbial degradation, using microbial secreted cellulaseand other auxiliary enzymes destruction of the medicinal plants of the cell wall, so asto achieve the purpose of the release of cell contents. Access to information throughthe final selection of Bacillus subtilis as fermentation. Through the screening of highyield strains of fermentation experiments of traditional Chinese medicine, andultimately determine the cellulase produced by Bacillus subtilis of most conditions.The results were as follows:1, the feed commodities Bacillus subtilis rejuvenation,30primary strains isobtained through the experiment of transparent circle, in after determination of thecarboxymethyl cellulase activity and filter paper enzyme activity assay, compoundsieve to strain the final. 2, by the screened strains of Panax notoginseng fermentation processing, by highperformance liquid chromatography analysis confirmed that the strain cansignificantly improve the release of Panax notoginseng contents.3, on enzyme production conditions of the strain were optimized. Identified theenzyme producing bacteria in most conditions, culture medium carbon source ofsodium carboxymethyl cellulose, peptone and yeast extract (you can also select twokinds of nitrogen mixture) the selection of nitrogen source, carbon source added2%,nitrogen source added4%. The fermentation inoculum size should be4%,fermentation temperature is381DEG C, the reaction of PH neutral PH7.0,fermentation period is48days. Through the study of the strain producing cellulaseproperties to determine the activity of the enzyme optimum temperature is55DEG C,the optimum pH was7.0.. experiments also show the stability of the enzyme is better,at the optimal temperature and the most suitable pH near enzyme activity had noobvious fluctuations.