| Sensorineural hearing loss (SNHL) is one of the most common departments ofENT diseases. The lesions are mainly concentrated in the inner ear cochlear screwdevice and the auditory center of the brain, with hair cells or auditory neuropathy andhearing loss or deaf as the main features. In recent years, in the pathogenesis of many,the nerve inflammation and oxidative stress damage have become the hot research inrecent years. The oxidative damage caused by the increase of free radicals, and thechanges of pro-inflammatory and neural regulatory factors will occur after theoccurrence of SNHL. Studies have proved that drugs for antioxidant and regulatinglevels of pro-inflammatory factors have protective effects for gentamicin inducedSNHL. Therefore, this experiment was designed to explore whether rlz-8has theprotective effect and the possible mechanism of SNHL.Lingzhi (also called Ganoderma lucidum, Reishi, Munnertake, Sachitake andYoungzhi, etc.), known as “immortality”, basidiomycotina, is China’s traditionalvaluable herbal medicine, have the effect of nourishing and strengthening the body asdescribed by “Shennong bencaojing”. Restructuring of Ganoderma lucidumimmunomodulatory protein which used in this experiment is made use of geneticengineering, recoding the gene of LZ-8and make its secretion and expression inpichia, then through pilot fermentation and purification, end up with high purity andhigh stability of the protein. rLZ-8still inherited the LZ-8immune activity which canpromote peripheral lymphocyte proliferation, inhibit allergies, rejection, promoteadhesion factor secretion and antitumor activity.42guinea pigs which used in this experiment were randomly divided into sevengroups with6guinea pigs in each group: physiological saline group, model group, Ginaton group, puerarin group, rLZ-8low dosage group, rLZ-8middle dosage group,rLZ-8high dosage group. SNHL model was made by muscle injection of gentamicin(GM)100mg/kg/day, for14consecutive days, and before the physiological salinegroup and the model group both received intrapulmonary injections of physiologicalsaline, Ginaton group received intrapulmonary injection of Ginaton14mg/kg,puerarin group received intrapulmonary injection of puerarin10mg/kg, rLZ-8low,middle, high dosage group received intrapulmonary injection of rLZ-828μg/kg、56μg/kg、112μg/kg. The administration lasted till the last day that the GM beinginjected. During the treatment, guinea pigs were weighed and the results wererecorded. Each group was performed behavioral experiments：Preyer’s reflex. At thefirst and the last day that guinea pigs received GM injection, each group wasperformed Auditory Brainstem Response（ABR）test. After behavioral experiments,biochemical kits were used to assay the activities of catalase (CAT), malondialdehyde(MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and nitricoxide synthase (iNOS); ELISA method were used to detect the Interleukin-1β (IL-1β),Interleukin6(IL-6),Tumor Necrosis Factor-α (TNF-α).Behavioral experiments shown that rLZ-8can reduce the hearing damage of GMand improve the guinea pig outer auricle state; ABR results show that rLZ-8canreduce the increasing threshold shift of guinea pigs; HE results show that rLZ-8canprotect the Corti organ; Biochemical kit results show that rLZ-8can significantlyenhance the activities of CAT, SOD, GSH-Px, iNOS and reduce MDA content; ELISAresults showed that rLZ-8can reduce IL-1β, IL-6, TNF-α content.In conclusion, the present study demonstrated that rLZ-8can protect guinea pig’shearing performance from the SNHL caused by GM，The above data can bepreliminary showed that rLZ-8is a kind of potential that can be used in the clinicaldrug for the treatment of SNHL. |
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