Effect Of Oridonin-mediated Hallmark Changes On Inflammatory Pathways In BxPC-3Cells

Objective To investigate the effect of oridonin-mediated hallmark changes on inflammatory pathways in BxPC-3cells, and further find out the relationship between oridonin-induced inhibition effect and inflammatory factors and its signal pathway. Exploring the inhibitory mechanism of oridonin on pancreatic cancer is to provide oridonin not only possible anti-tumor accesses but also favorable clinical data.Methods BxPC-3cells were cultivated sterilely, and the proliferation inhibition efforts of oridonin were tested by MTT array in cells. We obtained data of the lowest concentration, the best concentration, and IC50for BxPC-3cells. BxPC-3cells were treated by0,8,16and32μg/mL oridonin for24h respectively. And the effects of oridonin on inflammatory factor in BxPC-3cells were measured by ELISA. BxPC-3cells were treated by0,8,16and32μg/mL oridonin for24h respectively. The hallmark changes of BxPC-3cells were observed by Western Blot. The effects of oridonin on AP-1/NF-κB, IL-6/STAT3, and TGF-β/Smads signal pathway in BxPC-3cells were observed by Western Blot and RT-PCR. And the effects of oridonin on nuclear transcriptional factor IL-33in BxPC-3cells were observed by immunofluorescence staining under0,8, and32μg/mL oridonin treatment.Result MTT array showed that oridonin inhibited the proliferation of BxPC-3cells in a manner as dose-dependent. The lowest observed effect concentration of oridonin was8μg/mL, the optimal concentration was32μg/mL, and the IC50was19.32μg/mL in BxPC-3cells. Compared with control group, the expression of proteins within tumor hallmarks in BxPC-3cells were down-regulated by the oridonin at the concentration of8μg/mL,16μg/mL and32μg/mL in different degrees. ELISA analysis showed that the inflammatory factor in BxPC-3cells were decreased by oridonin at8μg/mL,16μg/mL,and32μg/mL in a dose-dependent manner, compared with control group. Compared with control group, the expressions of protein or mRNA within AP-1/NF-κB, IL-6/STAT3, and TGF-β/Smads signal pathway in BxPC-3cells were down-regulated by the oridonin in different degrees. Compared with control group, the expressions of nuclear transcriptional factor IL-33protein in BxPC-3cells were down-regulated by the oridonin at the concentration of8μg/mL, and32μg/mL in different degrees.Conclusion①Oridoriin could inhibit the proliferation, invasion and metastasis, angiogenesis of BxPC-3cells.②ridonin could down-regulate the secretion of inflammatory factors in BxPC-3cells.③ridonin could down-regulate the expression of proteins related with inflammatory factor signal pathway in BxPC-3cells. Then the inflammatory factor signal pathway may be influenced by oridonin.