Studies On The Bioactive Metabolites Of Sponge-Dderived Fungal Strains Hypocreales Sp. And Penicillium Sp

Sponge-derived fungi increasingly draw attention as an important source of novel bioactive secondary metabolites. To investigate the metabolites of the sponge-derived fungi and search for new pharmaceutical lead compounds, two fungal strains HLS-104and HLS-216, which were isolated from a marine sponge Gelliodes carnosa collected from South China Sea. were cultivated in rice solid medium. The cultures were extracted with EtOAc and the extrats were further purified by various chromatographic methods. The isolated compounds were elucidated on the basis of spectroscopic analysis and chemical methods as well. Some of them with sufficient amount were assayed for their bioactivities.There are8compounds isolated from the fungus HLS-104, including4sesquiterpenes,3esters and1sterol. These compounds are elucidated as10-hydroxy-4(5)-muurolen-3-one (1),6,10-dihydroxy-4-en-3-one (2),9,10-dihydroxy-4-en-3-one (3), nectriapyrone (4),2H-pyran-2-one,6-pentyl-5,6-dihydro-(5), bis (2-ethylhexyl) phthalate (6), ergosterol (7). Compounds2and3are new ones.Five compounds were obtained from the fungus HLS-216, including1secalonic acid,1amide,1nitrogen-containing compound,1esters, and1alkaloids, they are listed as follows:secalonic acid (8), oxeline (9),4-hydroxyl cinnamon amide (10),4-hydroxy-benzeneacetic methyl ester (11),4-hydroxy benzeneacetonitrile (12).Anti-tumor, anti-inflammatory, and anti-virus activity screening were carried out in the study, the results showed that compound9has significant anti-tumor activity; compound5can inhibit the generation of NO in mouse peritoneal macrophages.