Lactobacillus DM8909Cell Wall Peptidoglycan Of Vaginal Infection In Mice Immune Regulation

Objective:Cytokines vaginal tissue of mice was measured to reflect the peptidoglycan of Lactobacillus vaginal immune regulation.Methods:By trichloroacetic acid (TCA) method is used to get the lactobacilli DM8909peptidoglycan extract; Take six to eight weeks, A total of36kunming mice, were randomly divided into six groups, each group of6, only group A as the normal control group, group B with antibiotics and e. coli EPEC104cause vaginal infection model in mice after given different disposal, including B1for illness group, made directly executed after vaginal infection model in mice take its cytokines determination of vaginal tissue, B2for natural recovery group, make vaginal infection does not offer any disposal model after6days later executed mice take its vagina determination of cytokines, B3for physiological salt spa group, make vaginal infections after model to20v1saline injected into mice vaginal, continuous6d, once A day, death take its vaginal tissue in mice after determination of cytokines, B4in the treatment of lactic acid bacteria group, make vaginal infections after model give Howard’s lactic acid bacterium DM890920v1injected into mice vaginal bacteria liquid, for six consecutive days, once per day, death take its vaginal tissue in mice after determination of cytokines, B5for lactobacillus peptidoglycan treatment group, make vaginal infections aftermodel give Howard’s lactic acid bacterium DM8909peptidoglycan20vl, six consecutive days, once per day, death take its vaginal tissue in mice after determination of cytokines; By ELISA (enzyme-linked immunosorbent method) to determine vaginal local tissue cells in mice factor the level of IL-2and IL-10.Result:l.Each experimental group compared with IL-2in normal control group, the group level of IL-2significantly increases, the difference was statistically significant (P<0.01); Natural recovery group had elevated levels of IL-2, the difference was statistically significant (P<0.01); Physiological saline treatment group had elevated levels of IL-2, the difference was statistically significant (P<0.01); Lactobacillus treatment group IL-2level slightly decreased, there was no statistically significant difference (P>0.05); Lactobacillus peptidoglycan treatment group also slightly reduce the level of IL-2, there was no statistically significant difference (P>0.05)2.Each group experimental group and the comparison of IL-2:normal control group significantly lower levels of IL-2, the difference was statistically significant (P<0.01); Natural recovery group of IL-2level slightly decreased, there was no statistically significant difference (P>0.05); Saline treatment group (IL-2level slightly decreased, there was no statistically difference (P>0.05); Lactic acid bacteria treatment group significantly lower levels of IL-2, the difference was statistically significant (P<0.01); Lactobacillus peptidoglycan IL-2significantly reduce either treatment group, the difference was statistically significant (P<0.01)3.Lactobacilli treatment group and lactobacillus peptidoglycan treatment group level of IL-2comparison there was no statistically significant difference (P>0.05)4.Each experimental group and the comparison of IL-10in normal control group, the group IL-10levels significantly lower, the difference was statistically significant (P <0.01); Natural recovery group IL-10levels decrease, the difference was statistically significant (P<0.05); Saline treatment group IL-10levels were lower, the difference was statistically significant (P<0.05); Lactic acid bacteria treatment group IL-10levels were slightly lower, there was no statistically significant difference (P>0.05); Lactobacillus peptidoglycantreatment group IL-10levels slightly higher, there was no statistically significant difference (P>0.05).5.IL-10in each experimental group and disease group:normal control group IL-10levels increased significantly, the difference was statistically significant (P<0.01); Natural recovery group IL-10levels were slightly higher, there was no statistically significant difference (P>0.05); Physiological saline treatment group IL-10levels slightly higher, there was no statistically significant difference (P>0.05); Lactic acid bacteria treatment group IL-10levels increased significantly, the difference was statistically significant (P<0.01); Lactobacillus peptidoglycan treatment group IL-10levels also increased significantly, the difference was statistically significant (P<0.01). 6.Lactobacilli treatment group and lactobacillus peptidoglycan treatment group IL-10level comparison there was no statistically significant difference (P>0.05)...