| Objectives:To analyze the feature of epidemiology and clinical characteristics of Group A rotaviruses (GARV), Human Bocavirus (HBoV), Human Calicivirus (HuCV), Human Astrovirus (HAstV), Enterovirus (HEV) and Human Adenovirus (HAdV) in Lanzhou area of China from July2012to June2013, and to provide scientific data for the prevention and control of children diarrhea.Methods:A total of331stool samples were collected from hospitalized children less than five years with acute infection diarrhea, in Department of Pediatrics, the First Hospital of Lanzhou University, between July2012and June2013. Personal information and Clinical data were collected from all children. Group A rotavirus was identified by enzyme immunoassay (EIA). Rotavirus G serotype, P serotype and HBoV were confirmed by nested-PCR. HuCV, HAstV, HEV and HAdV were detected by reverse transcription-Polymerase Chain Reaction (RT-PCR) or PCR.The positive stool samples were sequenced.Results:1.Of the331stool samples,45.32%were rotavirus-positive. GARV was the most prevalent virus. The results indicated that G9(84.00%) has gotten the highest detection rate for the first time, followed by G3(12.67%), G1(1.33%), G2and G1+G9(1.33%). No G4was detected. P[8] accounted for the most majority (96.97%), P[4] and P[4]+P[8] type were2cases (1.33%), P[6] was1cases (0.67%).The most common G/P combination identified was G9P[8], followed by G3P[8]. The most common co-infections were Rotaviruses and Bocavirus. GARV was no obvious distribution of seasons, peaked in March. The median age was9months (rang,0month to28months). Of all episodes of rotavirus diarrhea,96.0%occurred during the frist18months, peaking at7-12months. Compared with non-GARV, GARV infections can be associated with severe clinical presentation, including vomiting (66.0%), fever (67.3%), frequency of diarrhea(>5times/day)(57.3%) and frequency of vomiting(>3times/day)(40.4%).2.Among the331stool samples,14.8%(49/331) were bocavirus-positive. We found human bocavirus1,2,3and4at respective26,15,7and1cases. The seasonal distribution of HBoV was throughout the year. HBoV was mostly found in children aged from7to12months with a median age was9months. The children under2years old were the most susceptible population for HBoV infection (93.88%).71.3%of the mixed infections was detected and the majority were rotaviruses. There was no statistic difference in incidence of fever and vomiting with HBoV infection. A rare virus of HBoV4(LZFB086) was identified, which showed highest level of nucleotide sequence identity with one Thai HBoV strain (JQ267789) at99.0%. No HBoV2B was found.3. Laboratory investigation revealed4predominant viruses:calicivirus, astrovirus, enterovirus and adenovirus. After GARV and HBoV, HuCV(12.99%) was the third most frequently etiologic agents of viral diarrhea, including NoV (76.74%) and SaV23.26%). The predominant type of HuCV was GII/4(48.48%), followed by non-typeable for Gâ…¡ (24.24%). HAstV1(88.46%) was detected mostly in all Human Astrovirus positive cases. Human enterovirus was present in5.74%of all cases,63.16%was Poliovirus and36.84%was Coxsackievirus. No Echovirus was found. Fifteen of human adenovirus were detected, belonged to three subgenera (F, A and C) with five serotypes HAdV41(7), HAdV40(3), HAdV31(3), HAdV2(1) and HAdV5(1). Enteric adenovirus was the dominant serotype in these serotypes.Conclusion:1. Group A rotavirus was a major pathogen of diarrhea in children in Lanzhou area. G9got the highest detection rate for the first time. The introduction of effective G9vaccines is necessary.2. HBoV1was a major pathogen of HBoV in Children cases in Lanzhou. The HBoV4was detected in feces for the first time in China. The data presented suggests that HBoV is not a causative agent of gastroenteritis. |
PDF Full Text Request