Establishment Of Diagnostic Model Of Serum Protein Finger-print For Noise-induced Hearing Loss Susceptible Individual In Tunnel Troops

Among occupational diseases, the morbidity of noise-included hearingLoss (NIHL) ranks the second just after pneumoconiosis. Troops responsible formilitary sap construction of PLA distribute to tunnel in enclosed environmentyear by year. The noise level in such environment always exceeds the safetylevel, which might easily cause NIHL. It is reported that the susceptibility ofNIHL exits and varies among different individuals. Therefore it is meaningful tostudy the screening measures of susceptible individual to NIHL in troopsworking in military tunnel for saving battle effectiveness and realizing theobjective of building a strong army. Up to now, most researches on NIHLsusceptibility focused on a sole gene and there is no effective screening methodof susceptible individual. Protein is the main element for organism performingphysiological function. The research on selection measures of NIHL susceptibleindividual should focus on finding a set of proteins or peptide fragment withdifferences and establish diagnostic model based on various characters to avoidcontradiction of sensitivity and specificity caused by detection with sole gene orsimple superposition of several genes. Through research on noise exposure leveland serum differential protein of soldiers from a certain tunnel constructiontroop, this paper preliminarily studied the screening method of NIHL susceptibleindividual by using diagnostic model of serum protein finger print.Part One: Survey on condition of distribution of NIHL susceptible individual from a certain tunnel construction troop.During this research, epidemiological survey on hearing function of296soldiers from one tunnle construction troop was carried out by the means ofquestionnaire and pure tone audiometry. Eight soldiers of NIHL were found withpercentage of2.70%(8/296), and64ones of high frequency hearing loss werefound with percentage of23.65%(70/296);83ones as early observation objectswere found with percentage of28.04%(83/296). According to the result of fieldresearch, all constructors are divided into9groups and then their noise exposurelevel was measured by personal noise exposure meter respectively. The resultshowed that the average value of Leq.8his88.90dB（A）which exceeded thelimitation of national standard. Except cement feeders and weak noise workers,the noise exposure level of the others exceeded safety level specified in nationalstandard. According to homenergic effect theory, the linear prediction modelbased on relationship between cumulative noise exposure (CNE) and averagehigh frequency hearing threshold was established. Then distribution diagram ofNIHL susceptible individuals was composed based on above model. It wasfound that susceptibility distribution of individual to noise exposure isleft-skewed with different value between-20~59dB and peak value between-5~-1dB. This provided a feasible program to select NIHL susceptible individualfor the next step.Part Two: Establishment of diagnostic model of serum protein finger-print ofNIHL susceptible individual in a certain tunnel troop.Based on the results of the first part, MALDI-TOF/MS and ClinPro-Toolssoftware were used to screen differential proteins in serum from above soldiers.The result showed that two polypeptides of statistical significance were found.Their molecular weight was1083.57Da and1061.68Da. Both of thepolypeptides were down-regulated, which might become the biomarkers oftunnel NIHL susceptible individuals. The diagnostic model of of NIHL susceptible individuals was established and its recognition rate was as high as91.67%.This research first measured noise exposure level in the workingenvironment of a certain military tunnel construction troop by using individualnoise exposure meter. The result showed that noise exposure level of most ofworker of this troop exceeded the national standard. To decrease NIHLmorbidity, individual protection shall be reinforced. The linear prediction modelof high frequency hearing threshold for the soldiers was first established.Furthermore, this research also studied the expression of differential proteins ofNIHL susceptible individuals. The protein finger-print diagnostic model ofNIHL susceptible individual was first established which provided valuable datafor exploring feasible methods to screen millitary tunnel NIHL susceptibleindividuals in the future.