Study On Correlation Between Expression Of Human Interleukin1,6in Involved Disc And Degree Of Discogenic Low Back Pain

Objective：To explore the relationship of the expression of interleukin1,6(Interleukin1,6IL-1,6) in the discogenic low back pain patients andthe grade evaluated by modified JOA score. Methods：30cases fromthe same race and district who suffered from discogenic low back painbetween45-65years of age were included into this study. All patientswere independently diagnosed by three different spine surgeons, andagreed by Hospital Ethics Committee. The inclusion criteria were:repeated low back pain more than3months, no response to non-steroidalanti-inflammatory drugs for two months, disc degeneration withoutherniation, obvious instability based on imaging data. The inclusioncriteria were: no history of low back pain within one year, vertebralfractures required surgical treatment, no disc degeneration based onimaging data. The degree of low back pain wasevaluated by modified Japanese OrthopedicAssociation scores (JOA).Thehighest was29points and the lowest was0points. The lower scoresindicated, the more servere back pain. The tested disc sample was singledout with homemake device which located the center of involved disc. The specimen at the center of disc in control group was harvested byanterior approach.The expression of IL-1and IL-6in disc was observedand calculated by immunohistochemical method and western blotrespectively. All the value were expressed by mean±SD and therelationship of the expression of interleukin1,6(Interleukin1,6IL-1,6)in the discogenic low back pain patients and the grade evaluated bymodified JOA score was analyzed by SPSS19.0statistical analysissoftware and Quantity One4.62. Results：The immunohistochemicalresults revealed that stained positive disc cells scattered in the field of themicroscope are mainly fibroblasts and cartilage cells. The cytoplasm wasbrownish-yellow colored and nucleus was pale blue. The expression ofIL-1and IL-6was12.509±0.845and32.965±0.224in the experimentalgroup and2.564±0.172and6.402±0.195in the control group, theexpression of IL-1and IL-6in the experimental group was higher than itin the control group.The expression levels of IL-1and IL-6was1.509±0.829,0.965±0.237in the experimental group and0.464±0.132,0.502±0.135in the control group tested by western-blot semi-quantitativeassay respectively, and there was significant difference between theexperimental group and the control group (P<0.05). The linearcorrelation analysis results of IL-1levels in experimental group and JOAscore revealed that the difference was statistically significant withr=-0.880, P=0.0005and P<0.05and indicated that IL-1levels and low back pain JOA score were negatively related. The linear correlationanalysis results of IL-6levels in experimental group and JOA scorerevealed that the difference was statistically significant with r=-0.890,P=0.0042and P <0.05and indicated that IL-6levels and low back painJOA score were negatively related. Conclusion：IL-1and IL-6in bothnormal and degenerated intervertebral discs are expressed, but itsexpression intensity in the degenerative group is significantly higher thanit in the normal group. The expression intensity of IL-1and IL-6wasnegatively correlated with JOA scores, the higher of IL-1and IL-6expressed, the lower the JOA scores were. This maybe suggest that IL-1and IL-6may play an important role in the mechanism of discogenic lowback pain.