Research On The Relation Between Genes Associated With The JAK/STAT Signaling Pathway And The Pathogenesis Of Uygur Psoriasis In Xinjiang

Objective: To recognize the differentially expressed genes of JAK/STAT signaling pathway related genesby comparing the mRNA expression levels between the lesions of psoriasis vulgaris and normal skin tissuein Xinjiang Uygur. And then analyze this gene expression levels correlated with the pathogenesis ofpsoriasis.Methods: The skin tissue of this study object was collected, and the total RNA was extracted, the cDNAwas reversely. Then the JAK/STAT signalling pathway gene expression levels of the psoriatic tissue of thesix cases of patients (experimental group) and the normal control skin tissue of5cases (control group) wasdetected via RT2profiler PCR Array. Via online software for data analysis, the differentially expressedgenes of those two types of tissues in this pathway were screened, and there was statistical significancewhile P <0.05and the expression up or down more than two-fold. The group GATA3, CCND1and JAK1protein expression among45cases in the experimental group and21cases in the control group wasexamined by immunohistochemical approaches, and the data was analysed using SPSS17.0statisticalsoftware.Results:1.Compared with the normal skin issue,17genes were found totally among Xinjiang Uygurpsoriatic tissue which expressed differentially more than2fold and P <0.05.There were9genes expressedup-regulated, including: CXCL9(13.26-fold，P=0.0469)、FCGR1A(2.24-fold，P=0.0263)、IL2RA(3.27-fold，P=0.0396)、IL6ST(2.71-fold，P=0.0075)、IRF9(2.12-fold，P=0.0344)、JAK1(7.91-fold，P=0.0218)、JUN(3.25-fold，P=0.0029)、OSM(3.11-fold，P=0.0435)、STAT1(2.91-fold，P=0.0026); while8genes weredownward expressed, including: CCND1(2.49-fold， P=0.0036)、 F2(3.27-fold， P=0.0387)、GATA3(2.62-fold，P=0.0031)、PIAS1(2.58-fold，P=0.0045)、PRL(3.03-fold，P=0.0059)、PRLR(3.81-fold，P=0.0346)、SMAD1(2.02-fold，P=0.0021)、SMAD3(2.78-fold，P=0.0055).2. The expression levels ofGATA3and CCND1protein in tissues of patients with psoriatic lesions were markedly reduced, and JAK1protein expression level was highly increased. Referred to the normal group the differences werestatistically significant (P<0.05), and consistent with the outcome from RT2profiler PCR Array.Conclusion: In the JAK/STAT signaling pathway, those multiple gene expression abnormalities such asJAK1, CCND1, STAT1and GATA3, may affect the occurrence and the development of psoriasis. Thesegenes involve cell growth, proliferation, apoptosis, transcriptional regulation, and other aspects offunctionalities, possibly participate in the pathogenesis of psoriasis, which can be recognized as a candidatesusceptibility gene of psoriasis. The systematic study of the related genes functions in JAK/STAT signaltransduction pathway in psoriasis can provide a theoretical basis for further elucidating the pathogenesis ofpsoriasis and developing new medicine targets.