Roles For PI3K/Akt Pathway In Impact Of The Expression Of Collagen Ⅰ、Ⅲ For Hepatic Stellate Cells By Hydrogen Sulfide

Objective:To investigate the effects of hydrogen sulfideon on rats hepatic stellate cells PI3K/Aktpathway. To investigate the Roles for PI3K/Akt pathway in impact of the expression of collagen IⅢ for hepatic stellate cells by Hydrogen sulfide.Method:（1）In vitro culture rats hepatic stellate cells(HSC-T6) and dispose the cells by50μmol/lsodium hydrosulphide which is the exogenous hydrogen sulfide donor.Western blot was used tocheck the expression of PI3K、p-Akt、GSK-3β and Bad（.2）In vitro culture rats hepatic stellatecells(HSC-T6) and dispose the cells by hydrogen sulfide,in the same time,give the cells specificinhibitors of PI3K/Akt pathway. Hepatic stellate cells can be divided into5groups，Normalcontrol group （N）； Hydrogen sulfide pretreatment group (S), given50μmol/l sodiumhydrosulphide (NaHS) pretreatment; Group D, dimethyl sulfoxide solute group (DMSO), givesthe final concentration of1‰DMSO medium; LY294002inhibitor group (LY), give contains50umol/L LY294002and1‰DMSO medium intervention cells; LY antagonism hydrogen sulfide(LY+S group), to contain50μmol/l LY294002, NaHS final concentration of50μmol/l, DMSOmedium intervention cells of the final concentration of1‰. RT-PCR was used to check theexpression of mRNA for collagen Ⅰ and collagen Ⅲ, Western blot was used to check theexpression of PI3K and p-Akt which were the upstream protein of PI3K/Akt pathway.Result:（1）Compared to the normal controlgroup, the expression of PI3K and p-Akt was elevated(P <0.05), the expression of GSK-3β and Bad was not have obvious difference(P>0.05).（2）Compared to the normal control group, the expression of collagen Ⅰ、 Ⅲ and PI3K、p-Akt waselevated in hydrogen sulfide group(P<0.05).Compared to the hydrogen sulfide group，theexpression of collagen Ⅰ、 Ⅲ and PI3K、p-Akt was decreased in the group which give thehydrogen sulfide and inhibitors at the same time（P<0.05）.Conclusion:（1）Hydrogen sulfide can activate PI3K/Akt pathway on rats hepatic stellate cells.（2）Hydrogen sulfide can activate PI3K/Akt pathway and elevated the expression of collagen I、 Ⅲon rats hepatic stellate cells...