| Objective: through the establishment of model of mesangialproliferative glomerulonephritis rats, to explore the mechanism YangyinZhixue Fang, detection of nuclear factor-κ B (NF-κ kidney B), B celllymphoma/leukemia-2(Bcl-2) expression level, and provide objectivebasis for clinical applicatioMethods:60Wistar rats. Were randomly divided into blank controlgroup, model control group, positive drug control group hematuriacapsule, Yangyin Zhixue Fang low dose group, middle dose group,Yangyin Zhixue Fang Yangyin Zhixue high dosage group. Theintermittent oral administration of bovine serum albumin, subcutaneousinjection of Freund’s adjuvant, tail injection of Staphylococcusenterotoxin B rat mesangial proliferative glomerulonephritis model, thelevels of urinary red blood cells eighth,11,14weeks the rats in eachgroup were determined, content determination of eighth rats for14weeks,24h urinary protein, on the fourteenth weekend detection the serum ureanitrogen (BUN) rats, serum creatinine (Scr), renal morphological changeswere observed under light microscope, the expression levels of NF-κBand Bcl-2assay in kidney tissue with immune groupResults:1to fourteenth weeks of Yangyin Zhixue high, medium andlow dose treatment group, the red cells decreased, the dose of YangyinZhixue Fang in the most significant effect of treatment group;2modelgroup of mesangial cells and matrix diffuse hyperplasia, hematuria Anncapsule group and Yangyin Zhixue Fang in the treatment group comparedwith the model of pathological damage group, glomerular mesangialvisible focal, segmental mesangial cell proliferation;3the model controlgroup Bcl-2, NF-κB expression increased significantly; hematuria capsulecontrol group, the treatment group was Yangyin Zhixue recipe expressiondecreased obviously Conclusion: Yangyin Zhixue can make MsPGN rat urine red bloodcell in urine decreased, can improve the pathological damage in themodel rat kidney tissue, expression can inhibit NF-κB and Bcl-2inmodel rats... |
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