| Objective: To compare antitumor activity of Xihuang dropping pill and XihuangWan based on the antitumor active component initial selection of Xihuang Wan, andcomparatively study the chemical constituents of Xihuang dropping pill and Xihuang Wanby modern instrumental analysis technology.Methods: The MTT assay on human tumor cell and anti-angiogenesis on zebrafishwere used to select the antitumor active component of Xihuang Wan. The MTT assay onhuman tumor cell,anti-angiogenesis on zebrafish and H22tumor-bearing mice in vivoanti-tumor function of the test were applied to evaluate the antitumor activity of Xihuangdropping pill and the results were compared with Xihuang Wan. The comparison ofXihuang Wan and Xihuang dropping pill chemical composition was studied by HPLC, GCand GC-MS methods.Results: The fat-soluble components were considered as the main antitumor activecomponent of Xihuang Wan, and ethanol extracts had the strongest antitumor effect.Xihuang dropping pill on MCF-7, T98g, Bv2tumor cell inhibition values of IC50were0.491mg/ml、1.145mg/ml、0.937mg/ml, Xihuang Wan IC50values were2.124mg/ml、1.952mg/ml、1.246mg/ml. Xihuang dropping pill production inhibitory effect on zebrafishembryonic intersegmental vessels of IC50value was18.07μg/ml, Xihuang Wan value ofIC50was20.66μg/ml.Xihuang dropping pill tumor inhibition rate up to76.93%in H22tumor-bearing mice. Xihuang dropping pill and Xihuang Wan mainly containingfrankincense and myrrh essential oil components, and Xihuang dropping pill contains thetotal content of index components and other components were more than Xihuang Wan.Conclusion: The fat-soluble components were considered as the main antitumoractive component of Xihuang Wan, and ethanol extracts had the strongest antitumor effect. Xihuang dripping pill could inhibit the proliferation of tumor cells and inhibitssignificantly zebrafish embryonic angiogenesis, and showed a better antitumor effect thanXihuang Wan.Xihuang dripping pill in H22tumor bearing mice with obvious antitumoractivity. Xihuang dripping pill and Xihuang Wan mainly containing frankincense andmyrrh volatile components, and new formulations of Xihuang dripping pill containedcomponents and the total content were more than Xihuang Wan. |
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