The Research Of Compounds In Hemp Pectin And Its Correlated Preliminary Pharmacodynamics

Hemp(Cannabis sativa.L) is the cannabis Branch (Cannabinaceae) of the genus Cannabis (Cannabis) annual herb. It is widely distributed in China’s Inner Mongolia, Ningxia, Qinghai, Gansu, Shanxi, Sichuan, Yunnan, Guangxi and other places. In history hemp has played an important role in an important source of fiber and food of the human need. In recent years, because of its huge economic and social value, pairs of the the research and development of of hemp are mainly concentrated in the parts of the hemp fiber, hempseed, flower, stalk, etc., but the research of Chinese hemp pectin is blank.We research the antibacterial active site of hemp to develop the drug application of hemp pectin. So this could avoid hemp pectin pollute the environment and at the same time do turning waste into treasure and make full use of hemp. Fill the blank of hemp pectin application to lay a good foundation is evidence-base.Different elution extracts were prepared using different macroporous resins D101, AB-8and SP-70. Cup-plate method and broth microdilution testing were used to determine the antimicrobial activities of the extracts against diverse bacterium, consisting of four kinds of Gram-positive bacteria, three kinds of Gram-negative bacteria and four kinds of fungus. When eluting ethanol concentration is30%, eluent of macroreticular resins D101shows no inhibitive effect for determined strain except for Bacillus coli; Eluting ethanol concentration is70%, the eluent eluted by three kinds of resins have powerful inhibitive effect for determined strain except for Staphylococcus aureus and fungus; when ethanol concentration is85%, eluent of resins D101has significant inhibition for four kinds of fungus, especially for Sabouraudites lanosus, but it is weaker than that of70%ethanol concentration.In order to investigate the chemical constituents in hemp pectin, we have adopted chemical methods. The constituents were extracted by70%-85%ethanol and purified by the macroreticular resin, Silica gel column chromatography, TLC. Spectroscopic methods were used to identify their structures. Seven compounds were obtained and identified as nemerosin (Ⅰ), vanillin(Ⅱ), scoparone (Ⅲ), rutin (Ⅳ), umbelliferone (Ⅴ), β-daucosterol (Ⅵ),β-sitosterol (Ⅶ).Biological thermokinetic method was been taken to study kinetics spectrum of the biological growth and metabolism of Escherichia coli, Staphylococcus aureus, Candida albicans under the action of nemerosin compounds. And then, kinetic parameters was been extrarcted to compare the difference of the action of different drug concentrations. In the same concentration, inhibition rate of nemerosin on Candida albicans growth was higher than the rest of the two. Hormesis effects were rendered at low concentrations, so its antibacterial effect and dose-effect relationships was been revealed.To establish an HPLC method for determinated the content of nemerosin in eluate of Cannabis Sativa is0.4%. The HPLC system consisted of Kromasil column C18(250mm×4.6mm,5μm); Acetonitrile:water (22:78); It was detected at314nm; Column temperature is room temperature; As mobile phase flow rate was1.OmL/min;under the chromatographic condition, control article nemerosin RT is7.672min. This method is convenient and accurate. It is suitable for the quality control of nemerosin of Cannabis Sativa L.