Effects Of ω-3Polyunsaturated Fatty Acids On Intestinal Microecology And The Injury Of Intestinal Epithelial Cell

Many studies show that fish oil with high content of ω-3polyunsaturated fatty acids (ω-3PUFAs) plays an important role in human health and disease. But the effects of fish oil with high content of ω-3PUFAs on gut microbiota, which are also known play a significant role in several human diseases, is not clear. In the present study we evaluated the effects of fish oil with high content of co-3PUFAs on gut microbiota. Changes in gut microbiota in ICR mice after supplementation of fish oil for15days was characterized using the hypervariable V3region of the16S rRNA gene-based polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) profiling, DNA sequencing, and phylogenetic analysis techniques.The results show that fish oil treatment resulted in a decrease in Helicobacter, Uncultured bacterium clone WD2_aaf07d12(GenBank:EU511712.1), Clostridiales bacterium, Sphingomonadales bacterium, Pseudomonas species Firmicutes and several uncultured bacteria. We can make a conclusion that Fish oil with high content of ω-3PUFAs are capable of producing significant changes in the gut microbiota that may, at least in part, explain the health benefits or injury induced by fish oil use.Environmental pollution and food safety problem has become important factors threating human health, and effect of heavy metals on human health is paid more and more attention, Intestinal epithelial cells is the main media connecting intestinal with envirment, and it is the first line of defense against pathogen invasion. Studies aboutω-3PUFAs and intestinal epithelial cells might be significant for revealing the relationship of ω-3PUFAs with intestinal health. In this paper, effects of ω-3PUFAs on injury of IEC-6cells induced by heavy metal and its mechanism were explored. The results showed that injury of IEC-6cells induced by heavy metal was inhibited by ω-3PUFAs, which might be through inhibiting expression of ROS, EGF and IL-6cells to achieve. We inferred that ω-3PUFAs suppressed oxidative damage and proinflammatory cytokine secretion in IEC-6cells induced by heavy metal.