The Relation Between HPV-16Infection And Integration And The Cervical Cancer

Objective:1To explore the role of HPV-16integration in the occurrence and development of cervical lesions by measuring the existential state of HPV16in cervical lesions;2To find out the relationship between the HPV mount and the cervical disease.Methods::1pretests:the mutiplix-PCR which can not only test the infection of HPV but also can test HPV16and its status is tested by HPV16, HPV18and HPV31single or mixed plasimids and some screening TCT samples.2from June,2010to December,2011150patients liquid-based cytology left samples are collected:They are positive in a corresponding period HC2tests and all with a final pathological result. Collect their basic information and analyze. These samples are divided into3groups,groupl:50samples from cervical cancer, group2:50samples from cervical lesions and group3:50samples from normal cervix.3Extract the whole genome of those samples. Multiplex PCR was adopted to assess the viral integration state of HPV16infection with specific primers for HPV16E6and E2genes. The correlation between existential state of HPV-16and the cervical lesions was analyzed.Results:1. The radio of integrated HPV-16is correlated with the severity of cervical lesions. Episomal ones are more common in normal group (73.5%),while in cervical cancer group it only took up4.8%; integration took up3%of normal group? integration ones are more common in normal pathological result (66.7%). There is significant difference in the integration rate among the3groups (X2=57.04, P<0·001), and the integration of HPV-16is positive correlation with pathological result (r=0.704; P<0.001).2The radio of integrated HPV-16is correlated with the severity of cytological result. Episomal ones are more common in WNL (64.52%), while integration took up70.83%of HISL ones. Episomal ones are more common in normal pathological result (64.52%), while integration took up70.83%of cervical cancer. There is significant difference in the integration rate among the3groups (X2=57.37, P<0·001), and the integration of HPV-16is positive correlation with cytological result (r=0.635, P<0.001).3. There is significant difference of HC2result among the3groups (ANOVA F=29.137, P<0.001), among each two groups P<0.001。There is significant difference in HC2degree result among the3groups (X2=52.85, p<0.001), and the HC2degree result is positive correlation with pathological result (r=0.589; P<0.001)Conclusion:1HPV16physical status shows difference in the3groups, so the intergradations of HPV16in TCT samples can predict the pathological result.2. HPV16physical status shows difference in different cytological result, so the intergradations of HPV16in TCT samples can predict the cytological result.3. The mount of HPV in cervical cancer TCT samples group is found to be higher than normal groups.