Application Of Microarray Analysis In The Research Of Gastric Cancer Genome

Background：As medical technology advances and people’s increasing emphasis,early diagnosis rate of gastric cancer increases, sophisticated methods of treatmentincreases. Despite the use of comprehensive measures such as chemotherapy andradiotherapy treatment to surgery, for patients with advanced gastric cancer, effect isstill not satisfactory. Cancer is a multi-gene diseases, genome-wide study of tumorgradually been paying attention, make clear the genetic structure of tumor cells,intervene cell gene expression is an important direction of cancer research treatment.Currently, there are fever genome-wide study on gastric cancer. Gene treatmenteffects have limitations. Microarray analysis can be a large-scale analysis of genomicdata, make clear the abnormal changes in gastric cancer genome, in order to achievetargeted and personalized treatment.Objective: Through the genome-wide high-resolution microarray analysis ofgastric specimens to make clear the gastric genome gene copy number abnormalitiesand the loss of heterozygosity.Analysis gene sequence in variation regions. Providenew markers for early diagnosis, as well as provide a new target for gene therapy.Methods: In this study, we collect the tissue samples of gastric cancer from theAffiliated Hospital which diagnosis is gastric cancer by the comprehensive clinical,radiological and pathological diagnosis. Extract its DNA, through the application ofCGH+SNP microarray chip analysis techniques to analyze the genome chromosomecopy number abnormalities and heterozygous deletions,to analysis the chromosomeabnormalities gene of copy number.To make clear the correlation with gastric cancer,to propose new tumor markers as well as pave the way for personalized treatment. Results:35cases of gastric cancer specimens have complexed loss and (or)amplification of copy number and heterozygous deletions, wherein gastric DNA1p,3p,4p,5q,6q,8p,9p,10q,11q,14q,15q,16q,17p,18p,19p,21q,22q, wholeY exist ingene copy number loss,1q,3q,5p,6p,7p,8q,9q,11p,12q,13q,17q,19q,20q, Xqpresence of the gene copy number amplification.Of which more than50%of casesfound the amplification of copy number in8q24.11q24.21,8q24.21q24.22,8q24.3,45%cases exists the loss of copy number in3p14.2. More specimens found the wholechromosome20copy number amplification and Y chromosome copy number loss ofthe whole, is a new direction for further research. At the same time we found thatsome cancer-related genes, such as FHIT, APC, DCC, WWOX etc. exist copy numberloss, and gene CDKs, EGFR,PIK3CA etc. exist copy number gain. And found thatloss of MTAP, NDUFA13gene copy number and the SET, RAP2B, SRC gene copynumber amplification has correlation with cancer of the stomach, At present, fewstudies of these genes related to gastric cancer, can be used as the direction of furtherresearch. Although many cases of gastric DNA samples exist heterozygous deletions,but we found less meaningful genes. PAX1studies have shown that it is associatedwith breast cancer,which can be combined with gastric cancer in the future grouping.Conclusions:1. We can find gastric DNA1p,3p,4p,5q,6q,8p,9p,10q,11q,14q,15q,16q,17p,18p,19p,21q,22q, wholeY exist in gene copy number loss,1q,3q,5p,6p,7p,8q,9q,11p,12q,13q,17q,19q,20q, Xq presence of the gene copy numberamplification.Of which more than50%of cases found the amplification of copynumber in8q24.11q24.21,8q24.21q24.22,8q24.3,45%cases exists the loss of copynumber in3p14.2. More specimens found the whole chromosome20copy numberamplification and Y chromosome copy number loss of the whole.2. We found that some cancer-related genes, such as FHIT, APC, DCC, WWOXetc. exist copy number loss, and gene CDKs, EGFR,PIK3CA etc. exist copy numbergain. 3.We found that loss of MTAP, NDUFA13gene copy number and the SET,RAP2B, SRC gene copy number amplification has correlation with cancer of thestomach.