The Association Between Genes Polymorphism Of IL-8or CD14and Necrotizing Enterocolitis

Background and Objective:Necrotizing enterocolitis (NEC)，which often occurs in the premature infant，isone of the most common cause of gastrointestinal-related mortality in the neonatalintensive care unit (NICU). UP to now，the pathogenesis of necrotizing enterocolitis(NEC) remains unknown, but it is probably a heterogeneous disease resulting frommultiple factors including prematurity， milk feeding， infection， microbialcolonization.Although the premature infants are given the same interventions,whetherto develop to NEC is unkown.The severity of the Illness and the effectivenessof the treatment is various. It prompt us some genetic factors play a role in thedevelopment of NEC.CD14is an important mediator of the body’s natural immune response system,which can be associated with bacterial lipopolysaccharide (LIPoPolysaeeharide, LPS)binding and activation of Toll-like receptor4(Toll-like receptors4, TLR4) signalingpathway, causing inflammatory cytokine expression level linking reaction.Chemokine can promote the white blood cells migrate to the area ofinflammation.IL-8is an important pro-inflammatory cytokine of chemokine.It havepowerful chemotaxis for neutrophils and lymphocytes, and adjust all kinds ofinflammation.Studies have shown that CD14, IL-8levels of NEC infants weresignificantly increased, suggesting that they may play a role in the pathogenesis ofNEC. The CD14promoter-159T/C and IL-8promoter-251A/T single nucleotidepolymorphism, can affect the expression of CD14and IL-8. This article is tells aboutthe association analysis of IL-8and CD14genes polymorphism with NECMethods:Sanger gene-sequencing method was used to re-sequence the IL-8promoter-251A/T and CD14promoter-159T/C polymorphism loci of28premature infants whowere diagnosed NEC in neonatal intensive care unit of The first Bethune hospital of Jilin University from February21,2014to March21,2015.These genes polymorphismloci were compared with41non-NEC premature infants.The test was used forstatical analysis.Results:1.The the frequency of CD14-159C/T genotypes was C/C （17.9%,5/28），C/T（35.7%,10/28），T/T （46.4%,13/28）in NEC group.It was C/C （29.3%,12/41），C/T （39%,16/41），T/T （31.7%,13/41） in Control group.There was no statisticaldifferences between the two groups(p>0.05).2.The the frequency of IL-8-251A/T genotypes was C/C （17.9%,5/28），C/T（35.7%,10/28），T/T （46.4%,13/28）in NEC group.It was C/C （29.3%,12/41），C/T （39%,16/41），T/T （31.7%,13/41） in Control group.There was no statisticaldifferences between the two groups(p>0.05).3.The frequency of CD14-159C/T allelic genes was C35.7%，T64.3%in NECgroup;C46.5%，T53.5%in Control group.There was no statistical differences in thefrequency of CD14-159C/T allelic genes between the NEC group and Controlgroup(p>0.05).4.The frequency of IL-8-251A/T allelic genes was A26.8%，T73.2%in NECgroup;A43.9%，T56.1%in Control group.The frequency of T allelic genes inNEC group was increased (p>0.05).Conclusions：1.Our studies show no association beween CD14-159C/T SNP and NEC.2. IL8-251A/T SNP may increase the risk of NEC in preterm infants.