The Role Of RelB In Prostate Cancer And The Discussing Of Its Mechanism

Objective This study is to clarify the role of RelB in prostate cancer and explore theinternal mechanism of those effects.Methods The expression of protein of the family of nf-kappa B in three prostatecancers was detected by Western blot. Establish a stable transfection of RelB-siRNA inDU145. The expression of protein of nf-kappa B family was dectected by Western blot.The changes of cell growth, migration ability and invasion ability were observed whichwas after RelB blocking in DU145by Real-time dynamic xCELLigence system. Theexperiences of cell cycle, cell proliferation and cell apopotosis in DU145which was afterRelB blocking were dectected by using flow cytometry. The related genes mRNA level ofDU145-control and DU145-siRelB was dectected by qRT-PCR. The migration ability inDU145which was after RelB blocking was observed by using scratch experiment. Theactivity of MMP2and MMP9in DU145which was after RelB blocking was dectected bygelatin MMP zymography.Results The expression of the protein of RelA, p50, RelB and p52in androgendependented prostate cancer cell DU145and PC-3was high persistent. The expression ofthe protein of RelB was highest in DU145. The plasmids of pSilencer3.1-siRelB andpSilencer3.1were stable transfected in DU145respectively, and we successfully got theexperimental group RelB lower expression DU145-siRelB and the control groupDU145-control. The expression of protein of other members of nf-kappa B family was nodifference after the blocking of RelB. The cell growth of DU145-siRelB was slower thanDU145-control. The ability of migration and invasion of DU145-siRelB was moredecreased than DU145-control. And the scratch experiment was also showed that theability of cell migration of DU145-siRelB was weaker than DU145-control. There is nodifference of the experiments of cell cycle and cell proliferation between DU145-controland DU145-siRelB. The apoptosis of DU145-siRelB cells was increased obviously thanDU145-control cells. The expression level of mRNA of the gene of bcl-2in DU145-siRelB was lower significantly than c cells, but the expression level of mRNA of the gene ofBcl-xl, ICAP1, Bax, A20, Bim and Mcl-1had no difference in DU145-control andDU145-siRelB cells. The expression level of mRNA and protein of the gene of ITGB1wasdecreased significantly in DU145-siRelB than DU145-control cells. The expression levelof protein of MMP2and MMP9was lower significantly in DU145-siRelB thanDU145-control cells, and the expression of MMP2and MMP9was lower significantly inDU145-siRelB than DU145-control cells by using gelatin MMP zymography.Conclusion In the androgen dependented prostate cancer cells, the expression level ofprotein of RelB was the highest in DU145cells. The cell growth and the ability ofmigration and invasion of DU145-control cells was more quickly than DU145-siRelB. Theabsence of RelB significantly inhibited the growth of the DU145prostate cancer cell line,and associated with apoptosis. The decreasing of bcl-2gene may be one of the potentialreasons. The absence of RelB significantly inhibited the migration and invasion of cancercells, the decreaseing of the expression of ITGB1gene and the diminished vitality ofMMP2and MMP9may be one of the potential reasons.