Experimental Study On The Effect Of Qian Lie Ling Capsule On The Inflammation Regulatory Network And Oxidative Stress Level In Chronic Prostatitis

[Objective]:To evaluate the effect of treatment that Qianlie Ling Capsules on chronic prostatitis in rats, to investigate the effect of Qianlie Ling Capsule on the regulatory network and oxidative stress level in chronic prostatitis and to provide experimental and theoretical basis for the development and application of traditional Chinese medicine for treatment of chronic prostatitis[Method]:60male rats, which were15months old and Weight250-350g, domesticating and feeding after one week,were randomly divided into6groups of10each (blank group, positive control group, model group,Qlian Lie Ling Capsule high dose group, Qlian Lie Ling Capsule medium dose group, Qlian Lie Ling Capsule low dose group), Each given25%urethane(1g/kg) anesthetized by intraperitoneal injection, open the abdominal cavity on the abdominal incision,pull out bladder and two sides of seminal vesicle, exposed the dorsal lobes of the prostate under aseptic conditions.The blank control group respectively was injected of normal saline0.1ml through bilateral dorsal lobe prostate, the other groups were injected with25%Xiaozhiling injection0.1ml, sutured tissue and recovered for1week.Method of administration:The blank group and the model group daily gavage once, saline content which were administrated equaled to the Chinese Medicine content of experiment group; The positive control group (Qianlietongyu capsule):each rat was orally given Qianlietongyu capsule suspension dose which was equivalent to20times adult dosage (weight60kg); Qian Lie Ling high, medium, low dose group:rats treated with Qian Lie Ling capsule suspension gavage, the dose which was equivalent to20times,10times,5times adult dosage (60kg), this three groups were daily gavage once; successive administration for30days, observed and recorded the weight and mental state changes in activity of rats every day,24h after the last administration, said the weight of each rat, took each rat venous blood3-5ml which was centrifuged and refrigerated, then decapitated each rat and excised prostate tissue.The excised prostate tissue was fixed with4%formaldehyde fixed liquid, ELISA method was used to analysis of the content of IL-6, ACP and SOD, MDA factors in the serum of the rats, S-P method was used to detect the changes of GSH, GST,and GSH-px factors in prostate tissue,electron microscope was used to observed the expression of apoptosis factor Bax, Bcl-2in prostate tissue.[Result]:Qian Lie Ling capsule can reduces the content of IL-6, ACP, MDA factors and increase the content of SOD in the serum of the prostatitis rats,and increase the content of GSH, GST, GSH-px factors in the prostate tissue of the prostatitis rats. Qian Lie Ling capsule also can up-regulate the content of Bax protein and down-regulate the content of Bcl-2protein. [Conclusion]:Qian Lie ling capsule has obvious therapeutic effect on chronic prostatitis rats, its mechanization relates to reduces the content of IL-6,ACP,MDA factors and increase the content of SOD factor in the serum of the prostatitis rats, and increase the content of GSH,GST,GSH-px factors in the prostate tissue of the prostatitis rats.Qian Lie ling capsule also can up-regulate the content of Bax protein and down-regulate the content of Bcl-2protein.