The Change Of Regulatory T Cells And Th17Cells In A Novel Mouse Severe Aplastic Anemia Model

Objective To approach the change of regulatory T cells and Th17cells in a novel mouse severe aplastic anemia (SAA) model induced by interferon-γ (IFN-γ) combined with busulphan, demonstrating the rationality of the model from immunology level.Methods We Used intraperitoneal injection with IFN-γ and intragastric administration with busulphan to set up the BALB/c female mice severe aplastic anemia model(associated group, n=15), with busulphan group (n=15), IFN-γ group (n=15) and normal group (n=15) as controls. After collecting the mononuclear cells in the peripheral blood and spleen of each group mice, we accessed the percentage of CD4+CD25+Foxp3+regulatory T cells and Th17cells in the mononuclear cells by using flow cytometry, as well as analysis.Results Both decrease in the percentage of the regulatory T cells in the peripheral blood and spleen was found in the associated group ((3.19±0.76)%,(4.77±1.05)%, respectively), having difference compared with other groups (P<0.01, respectively). The results also showed an increase in the percentage of the Th17cells in the peripheral blood and spleen of associated group ((2.07±0.12)%,(3.18±0.46)%, respectively), having difference compared with other groups too (P<0.05and P<0.01, respectively).Conclusions The decrease of regulatory T cells and the increase of Th17cells was found in the novel mouse SAA model induced by IFN-y combined with busulphan, demonstrating that this SAA model may be more close to the human immune-mediated marrow failure.