Effect Of RNA Inference On FoxQ1Gene In Non-small Cell Lung Cancer

Objective:To investigate the effect of inhibiting FoxQ1gene expression on the biological behavior of non-small cell lung cancer (NSCLC) by using a small interference RNA (siRNA) strategy.Methods:FoxQ1expression was evaluated in four human non-small cell lung cancer (NSCLC) cell lines(SPC-A-1, NCI-H1395, HCC827and A549) by Quantitative RT-PCR and Western blot. And then four segments of siRNAs targeting FoxQ1mRNA were designed by bioinformatics technology, and the no-sense control segment was also designed. The transfected efficiency was detected by Quantitative RT-PCR and Western Blot. Then CCK-8method was adapted to investigate the proliferation of SPC-A-1cells and the effect of chemotherapy sensitivity after transfection of FoxQ1siRNA. Moreover, we used flow cytometry and Western blot methods to examine the apoptosis cells after48h transfection of si-FoxQ1, compared with the control groups. In addition, the migration and invasion were examined by transwell chamber assays. Finally, we established subcutaneous tumor models to study the effect of FoxQ1on the growth of NSCLC cells in vivo. Results:1. The expression of FoxQ1gene and protein was significantly higher in SPC-A-1cell than the other three cells.2. The proliferation, migration and invasion ability of FoxQl silencing on SPC-A-1cell, decreased significantly than the control groups, while enhance the apoptosis (P<0.05)3. Tumorigenicity experiments showed that silencing of FoxQl decreased the tumorigenic capacity significantly than the control group (P<0.05).4. Chemotherapy drug sensitivity was enhanced significantly and the inhibition of tumorigenic capacity was increased when silencing the expression of FoxQ1.Conclusions:1. RNA inference on FoxQ1could reduce its expression in non-small cell lung cancer cells.2. Silencing FoxQl could inhibit the biological characteristics of SPC-A-1cells, while enhance drug sensitivity and induce apoptosis.3. Targeted intervention NSCLC by silencing FoxQ1, provide a theoretical and experimental basis for the targeted treatment of lung cancer..