Correlation Of UCP-1, ROS Level And Insulin Resistance And Diabetic Vascular Lesions In T2DM Patients

Objective To observe the change of serum UCP-1and ROS level in patients withT2DM with or without carotid plaque. Analyze the correlation between UCP-1,ROS and body fat parameters, HbA1c, fasting glucose, fasting insulin and HOMA-IR. Tostudy the roles of UCP-1and ROS in the development of T2DM and diabetic vascularlesions.Methods44patients with Type2diabetes without atherosclerotic macrovascularcomplications were set as Group B, while48diabetic patients with atheroscleroticmacrovascular complications (examined by ultrasound for detecting plaque formation ofcarotid artery) were set as Group C. And47healthy controls from the First AffiliatedHospital of Soochow University were enrolled in the study as normal control group (GroupA). T2DM was diagnosed with criteria of American Diabetes Association (Reference fromDiabetes Care published by ADA). Serum UCP-1and ROS levels were measured byELISA. Total cholesterol (TC), triglyceride (TG), high density lipoproteincholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), fasting bloodglucose (FBG), fasting insulin (FINS) and glycosylated hemoglobin (HbA1c) weredetected by the biochemical analyzer, and calculate insulin resistance index (HOMA-IR)with steady-state model.Results1. Compared with Group A, TG, TC, LDL-C levels were higher in Group Band Group C (P<0.01). Compare with Group B, TG, LDL-C levels were higher in Group C(P<0.05). There is no difference of HDL-C levels among three groups (P>0.05).2.Compare with Group A, HbA1c, fasting blood glucose, fasting insulin and insulinresistance index were higher in Group B and Group C (P<0.01), and there is no differenceof those in Group B and Group C (P>0.05).3. There is no statistical significance of UCP-1in3groups (P>0.05).4. Compared with Group A, ROS was higher in Group B and GroupC (P<0.01). And compared with Group B, ROS was higher in Group C (P<0.01).5. Singlefactor correlation analysis shows that UCP-1is negatively correlated with BMI (r=-0.343, P<0.05). And there is no significant correlation between UCP-1and TG, TC, HDL-C,LDL-C, HbA1c, FPG, FINS, HOMA-IR (r=-0.275,0.013,0.205,-0.148,-0.022,-0.224,-0.045,-0.074, P>0.05). ROS is correlated with BMI, TG, TC, LDL-C, HbA1C, FINS,HOMA-IR (r=0.519,0.298,0.333,0.315,0.352,0.511,0.533, P <0.05or P<0.01). There isno significant correlation between ROS and HDL-C, FPG (r=-0.039,0.249, P>0.05).6.Multiple liner stepwise regression analysis shows that BMI is a independent risk factor ofUCP-1(β=-0.041，P=0.031), and HOMA-IR is a independent factor of ROS (β=1.811，P=0.006).7. Single factor correlation analysis shows that ROS is negatively correlatedwith UCP-1(r=-0.411, P<0.05).Conclusion1. Hyperlipidemia may be involved in the development of diabetes andits vascular complications as a incidence risk factor.2. UCP-1was a related risk factor ofobesity.3. Oxidative stress is higher in obese diabetic patients and ROS may participate inthe occurrence and development of insulin resistance and diabetic vascular lesions.