The Effects Of Bromfenac Sodium On Human Lens Epithelial Cell Proliferation And Migration

Purpose:Posterior capsular opacification is a common long-term complication of modern cataract surgery. The purpose of this study is to evaluate the role that bromfenac sodium plays in the inhibition of PCO pathogenesis, including its effect on migration and proliferation of human lens epithelial cell in vitro.Methods:Human lens epithelial cells were either cultured for24h with different doses of TNF-a (5,10,15,20,25ng/ml), for choosing appropriate concentration of TNF-a to establish intraocular inflammation model. Cell proliferation and migration were respectively determined by methyl thiazol tetrazolium (MTT) assay and transwell chamber assay after incubation of HLECs with bromfenac sodium.Results:Human lens epithelial cells were cultured for24h with different doses of TNF-a(5,10,15,20,25ng/ml), the proliferation ratios were21.1%,60.9%,62.9%,64.6%,66.6%. According to the results of statistics, the group of TNF-a lOng/ml was chosen to establish intraocular inflammation model. Under the same concentration of TNF-a, human lens epithelial cells were cultured for24h with different doses of bromfenac sodium (20,40,60,80ug/ml). The difference between the groups was statistically significant (P<0.01). In the condition of inflammation, bromfenac sodium was an effective inhibitor of TNF-a induced HLECs proliferation and migration in vitro. All effects were dose dependent.Conclusions:In our study, bromfenac sodium strongly inhibited TNF-a induced HLECs proliferation and migration, and showed a significant dose-respone relationship. Thus, bromfenac sodium may have a potential inhibition of posterior capsule opacificatin (PCO) and needs further study.