The Study Of Apigenin On Human Colon Cancer HCT-8Cells Growth Inhibition And Effect In Induced Apoptosis

In recent years, the incidence of colon cancer is increasing year byyear. Asone of the common malignant tumors in our country, it seriouslydoes harm to human health. The clinical treatment of colon cancer is still inoperation, chemotherapy and other adjuvant therapy. Postoperativechemotherapy side effects are more, bringing some damage on the humanbody. Apigenin (apigenin, API) is a kind of flavonoids, abundant in fruits,vegetables, beans and tea. Previous studies found that apigenin hasanti-cancer,lipid-lowering, antihypertensive, vasodilator, anti-inflammatoryand other effects. Study on the anti tumor of digestive tract, celery,especially the research on colon cancer is rarely reported and thedestruction mechanism of colon cancer cells is not clear. This researchfocuses on human colon cancer cell line HCT-8observation of apigenininhibiting the growth of HCT-8cells and inducing apoptosis, and themechanism of cytotoxic effect of apigenin in human colon cancer HCT-8cells were discussed.Preliminary observation of apigenin on colon cancer cells have noinhibition of growth. This paper adopts MTT method to observe the effectof apigenin on growth and proliferation of colon cancer cells.The aholeexperiment were divided into negative control group,5μg·ml-1apigeningroup,10μg·ml-1apigenin group,15μg·ml-1apigenin group,20μg·ml-1apigenin group, respectively24h,48h,72h; adopted DNA gelelectrophoresis at different concentrations (5μg·ml-1apigenin group,10μg·ml-1apigenin group,15μg·ml-1apigenin group,20μg·ml-1apigeningroup and the negative control group) cells induced apoptosis of HCT-8; also using flow cytometry to detect the different concentration of celeryelement (10μg·ml-1apigenin group,20μg·ml-1apigenin group) cell earlyapoptosis induction of HCT-8. At the same time with differentconcentration of celery element (5μg·ml-1apigenin group,10μg·ml-1apigenin group,15μg·ml-1apigenin group,20μg·ml-1apigenin group) cellsdo for HCT-8after Caspase-3, Caspase-6enzyme activity were tested.Experimental results show that with the concentration of apigenin(5μg·ml-1,10μg·ml-1,15μg·ml-1,20μg·ml-1) increased, the apoptosis rateincreased gradually and showed a definite dose effect relationship;5μg·ml-1apigenin group、10μg·ml-1apigenin group、15μg·ml-1apigeningroup、20μg·ml-1apigenin group in HCT-8cells were detected in48hoursDNA Ladder, brightness group ladder was significantly lighter than thecontrol group, and it is related to drug measurement;15μg·ml-1apigeningroup and20μg·ml-1apigenin group can significantly induced apoptosisDNA fragments, and low concentration levels5μg·ml-1apigenin group,10μg·ml-1apigenin group) is not obvious; flow cytometry10μg·ml-1apigenin group,20μg·ml-1apigenin group,24hours early apoptosis ratewas (19.16±0.14) and (26.67±0.13)%; Caspase-3, Caspase-6enzymeactivity was significantly enhanced.In summary, this study found that apigenin on human colon carcinomaHCT-8cells inhibited the growth, apoptosis inducing effect. Caspase-3andCaspase-6activity was significantly increased, induced apoptosis of humancolon cancer cells may be accomplished by the way of apigenin. But itneeds further research whether there are other ways that apigenin caninhibit the proliferation of tumor cells.