The Level And The Clinical Significance Of Coronary Artery Disease Associated Genes In Peripheral Blood

Objective:Objective The aim of present work is to discover the relationship between coronary artery disease (CAD) and expressions of the four genes, CDK2A, CDK2B, MEF2A and VEGF.Methods:154CAD patients and123healthy control cases were enrolled in the experiments. All blood samples from patients and control cases were centrifuged for serum or extracted for RNA after red cell lysis. Four genes or proteins were detected by qualitative real time PCR or enzemy linked immunosorbent assay (ELSIA).Results:Expressions of CDK2A、CDK2B and MEF2A (0.01148±0.0021、0.003347±0.000437and0.3026±0.0356) genes in blood cells of CAD patients were significantly lower than that of control cases (0.04839±0.01563,0.007072±0.001157and0.8230± 0.0751, p<0.01). Meawhile, serum concentration of CDK2A, CDK2B, MEF2A and VEGF (121.6±7.955μg/ml,65.07±5.422μg/ml,6.513±0.832μg/ml and19.41±1.618μg/ml) of CAD patients all were lower than that of control cases (236.9±29.13μg/ml,155.7±22.52μg/ml,15.96±2.864μg/ml and39.87±4.544μg/ml, p<0.01).Conclusion:CAD patients had lower expression of CDK2A, CDK2B, MEF2A and VEGF than healthy people and this may be an indicator for CAD patient examination.