The Role And Mechanism Of MiR-224Targeting RASSF8in The Progression Of Cervical Cancer

ObjectiveTo confirm altered expression of miR-224in cervical cancer tissues and identify the predictive value of miR-224in the progression of cervical cancer.MethodsqRT-PCR were used to detect the expressions of miR-224.The clinicopathological parameters of these patients with cervical cancer were collected and analyzed. SiHa and CaSki cells were transfected with miR-224mimic as well as siRNA targeting RASSF8. MTT method, wound healing test, transwell assay, flow cytometry technique were used to detect the change of cellular proliferation, migration, invasion cell cycle and apoptosis separately. The expressions of RASSF8were detected by real time RT-PCR and immunohistochemical method. Luciferase activity reporter assays were used to identify RASSF8as the direct target of miR-224. Results1.miR-224is up-regulated in cervical cancer tissues comparing to normal tissues and the miR-224high expresser was correlated with poor prognosis factors.2.Over expression of miR-224promoted cell proliferation, migration and invasion but reduced the expression of RASSF8at protein levels.3.The expression level of RASSF8in the cervical cancer tissues is reduced compared with that in normal cervical tissues.4. The activity reporter assays revealed RASSF8is a direct target of miR-224.5. Knocking down of in SiHa and CaSki by siRNA inhanced cell migration, invasion but proliferation.Conclusions1.miR-224is up-regulated in cervical cancer tissues and positively correlated with poor prognosis factors.2.Over expression of miR-224via targeting RASSF8promotes the progression of cervical cancer.