Technique And Preliminary Quality Control Of Extracting The Yellow Paste

Objective: This topic according to the physicochemical properties andpharmacological effects of the Yellow paste of each medicinal material, make thepreparation process and quality control method of reasonable preliminary, the Yellowpaste made into the safe, stable, exact curative effect, good taste of food. Methods: Byusing the method of boiling water extraction, factors to decocting time, water multiple,decocting times, to yield as indexes, the screening of the Yellow paste the optimumextraction conditions of single factor and orthogonal test design; TLC identification, theeffective components of the qualitative identification of the Yellow paste medicinematerial, using ultraviolet visible spectrophotometry, total sugar content by0.2%anthrone-sulfuric acid solution, colorimetric determination of the Yellow pastePolysaccharide extract. Using the pycnometer method determination of yellow pasterelative density and insoluble. Acute toxicity experiment of yellow paste, LD50determination of yellow paste and1days maximum dosage. Results: To determinethe optimum extraction process of the Yellow paste was adding10times amount ofwater, soak30minutes, refluxing extraction for1hour, extracting for3times. Themeasured the Yellow paste of total sugar, polysaccharide content respectively is85.63%,31.7%. Preliminary made the quality of the Yellow paste requirements, a thickbrown semi liquid, no odor, burnt, no sugar crystallization. The Yellow paste averagerelative density is1.376±0.002(limited range1.35-1.40). Insoluble inspection inaccordance with the regulations, no coke debris and other foreign bodies. Yellow finepaste according to the toxicological evaluation standard, namely LD50>15000mg/kg,non-toxic substances. With roughly equivalent to human daily dose of (10g/kg)60times for drug. Continuous observation and no obvious toxic reaction. Conclusion: Thepreparation process is reasonable and feasible, reasonable and effective quality control,integrated the above detection index and parameters, can be formulated as a yellow paste extraction process and initial quality control reference, provide the basis for thedevelopment and utilization.